ArticleNanoscale2013
Robust and specific ratiometric biosensing using a copper-free clicked quantum dot-DNA aptamer sensor.
Article in Nanoscale, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Probing Glycan-Gold Nanoparticle Architectures: Glycan Type, Density, and Linker Length, Governing Multivalent Lectin Binding and Viral Inhibition.ACS applied materials & interfaces · 2026Article
- Sensing the Future-Frontiers in Biosensors: Exploring Classifications, Principles, and Recent Advances.ACS omega · 2024Review
- Article
- Article
- Quantum dots in biomedical applications.Acta biomaterialia · 2019Review
- Sensing with photoluminescent semiconductor quantum dots.Methods and applications in fluorescence · 2019Article
- The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection.The plant pathology journal · 2018Review
- Dissecting Multivalent Lectin-Carbohydrate Recognition Using Polyvalent Multifunctional Glycan-Quantum Dots.Journal of the American Chemical Society · 2017Article
- Ultraefficient Cap-Exchange Protocol To Compact Biofunctional Quantum Dots for Sensitive Ratiometric Biosensing and Cell Imaging.ACS applied materials & interfaces · 2017Article
- Quantum dots-DNA bioconjugates: synthesis to applications.Interface focus · 2016Review
- Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2016Article
- Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions.Angewandte Chemie (International ed. in English) · 2016Article
- Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection.Chemical Society reviews · 2015Review
- QD-Based FRET Probes at a Glance.Sensors (Basel, Switzerland) · 2015Review
- Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We report herein the successful preparation of a compact and functional CdSe-ZnS core-shell quantum dot (QD)-DNA conjugate via highly efficient copper-free "click chemistry" (CFCC) between a dihydro-lipoic acid-polyethylene glycol-azide (DHLA-PEG-N3) capped QD and a cyclooctyne modified DNA. This represents an excellent balance between the requirements of high sensitivity, robustness and specificity for the QD-FRET (Förster resonance energy transfer) based sensor as confirmed by a detailed FRET analysis on the QD-DNA conjugate, yielding a relatively short donor-acceptor distance of ~5.8 nm. We show that this CFCC clicked QD-DNA conjugate is not only able to retain the native fluorescence quantum yield (QY) of the parent DHLA-PEG-N3 capped QD, but also well-suited for robust and specific biosensing; it can directly quantitate, at the pM level, both labelled and unlabelled complementary DNA probes with a good SNP (single-nucleotide polymorphism) discrimination ability in complex media, e.g. 10% human serum via target-binding induced FRET changes between the QD donor and the dye acceptor. Furthermore, this sensor has also been successfully exploited for the detection, at the pM level, of a specific protein target (thrombin) via the encoded anti-thrombin aptamer sequence in the QD-DNA conjugate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.