ArticleJournal of visualized experiments : JoVE2013
Assembly of nucleosomal arrays from recombinant core histones and nucleosome positioning DNA.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Linker histones consolidate heterogenous nucleosome fiber contacts by linking together multiple nucleosomes.Nature communications · 2026Article
- Article
- Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes.Cell reports · 2022Article
- The Role of Non-Catalytic Domains of Hrp3 in Nucleosome Remodeling.International journal of molecular sciences · 2021Article
- Linker histone defines structure and self-association behaviour of the 177 bp human chromatosome.Scientific reports · 2021Article
- Analytical Ultracentrifugation (AUC): An Overview of the Application of Fluorescence and Absorbance AUC to the Study of Biological Macromolecules.Current protocols in molecular biology · 2020Article
- Article
- The transition structure of chromatin fibers at the nanoscale probed by cryogenic electron tomography.Nanoscale · 2019Article
- Single and double box HMGB proteins differentially destabilize nucleosomes.Nucleic acids research · 2019Article
- Mitotic post-translational modifications of histones promote chromatin compactionOpen biology · 2017Article
- Article
- Article
- Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Chromatin structure-dependent conformations of the H1 CTD.Nucleic acids research · 2016Article
- Nucleosomal arrays self-assemble into supramolecular globular structures lacking 30-nm fibers.The EMBO journal · 2016Article
- Touch, act and go: landing and operating on nucleosomes.The EMBO journal · 2016Review
- A quantitative investigation of linker histone interactions with nucleosomes and chromatin.Scientific reports · 2016Article
- Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation.Nucleic acids research · 2016Article
- In Vitro Chromatin Assembly: Strategies and Quality Control.Methods in enzymology · 2016Article
- Single molecule fluorescence methodologies for investigating transcription factor binding kinetics to nucleosomes and DNA.Methods (San Diego, Calif.) · 2014Article
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Authors and funding
6 authors.
Funding
Abstract
Core histone octamers that are repetitively spaced along a DNA molecule are called nucleosomal arrays. Nucleosomal arrays are obtained in one of two ways: purification from in vivo sources, or reconstitution in vitro from recombinant core histones and tandemly repeated nucleosome positioning DNA. The latter method has the benefit of allowing for the assembly of a more compositionally uniform and precisely positioned nucleosomal array. Sedimentation velocity experiments in the analytical ultracentrifuge yield information about the size and shape of macromolecules by analyzing the rate at which they migrate through solution under centrifugal force. This technique, along with atomic force microscopy, can be used for quality control, ensuring that the majority of DNA templates are saturated with nucleosomes after reconstitution. Here we describe the protocols necessary to reconstitute milligram quantities of length and compositionally defined nucleosomal arrays suitable for biochemical and biophysical studies of chromatin structure and function.
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