ArticleApplied and environmental microbiology2013
Occurrence of pepper mild mottle virus in drinking water sources in Japan.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
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Who cites it
118 citing papers in PubMed, 297 citations in OpenAlex.
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- First Detection of Hepatitis E Virus in Wastewater in Costa Rica.Food and environmental virology · 2026Article
- Genetic Evaluation of Pepper Mild Mottle Virus as an Indicator in Water Quality Monitoring and Human Fecal Contamination in Swedish Waters.Food and environmental virology · 2026Article
- Applying weighted quantile sum regression to wastewater surveillance: enteric viral mixtures and diarrheal disease trends in Costa Rica.Journal of water and health · 2026Article
- Comparative performance of activated sludge and waste stabilization ponds for the removal of pollutants and pathogens in full-scale wastewater treatment plants in Egypt.Scientific reports · 2026Article
- Evaluation of wastewater SARS-CoV-2 surveillance for monitoring COVID-19 trends and early warning in Sweden, 2023-2024.PloS one · 2026Article
- Unveiling the global urban virome through wastewater metagenomics.Nature communications · 2025Article
- Comparative Evaluation of Crassphage, Pepper Mild Mottle Virus, and Human Mastadenovirus as Indicators of Human Fecal Contamination in Surface Water.Food and environmental virology · 2025Article
- Utilities of PMMoV as an internal control for the building-level wastewater surveillance of SARS-CoV-2.Microbiology spectrum · 2025Article
- Partitioning and probe-based quantitative PCR assays for the wastewater monitoring ofJournal of water and health · 2025Article
- Optimizing solid-based methods for SARS-CoV-2 detection in wastewater: addressing PCR inhibition and variant challenges.Applied and environmental microbiology · 2025Article
- Hidden risks of biofilm-forming and multidrug-resistant Gram-negative bacteria in water vending machines.Antonie van Leeuwenhoek · 2025Article
- CrAssphage as a Human Enteric Viral Contamination Bioindicator in Marketed Bivalve Mollusks.Viruses · 2025Article
- Norovirus trends in British Columbia from 2021 to 2022: the relationship between wastewater surveillance and clinical outbreak data during the COVID-19 pandemic.Scientific reports · 2025Article
- Article
- Persistence of human Aichi virus infectivity from raw surface water to drinking water.Applied and environmental microbiology · 2025Article
- Optimized Aluminum Hydroxide Adsorption-Precipitation for Improved Viral Detection in Wastewater.International journal of environmental research and public health · 2025Article
- Spatiotemporal Variability of the Pepper Mild Mottle Virus Biomarker in Wastewater.ACS ES&T water · 2025Article
- Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method.Food and environmental virology · 2025Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pepper mild mottle virus (PMMoV) is a plant virus that has been recently proposed as a potential indicator of human fecal contamination of environmental waters; however, information on its geographical occurrence in surface water is still limited. We aimed to determine the seasonal and geographic occurrence of PMMoV in drinking water sources all over Japan. Between July 2008 and February 2011, 184 source water samples were collected from 30 drinking water treatment plants (DWTPs); viruses from 1 to 2 liters of each sample were concentrated by using an electronegative membrane, followed by RNA extraction and reverse transcription. Using quantitative PCR, PMMoV was detected in 140 (76%) samples, with a concentration ranging from 2.03×10(3) to 2.90×10(6) copies/liter. At least one of the samples from 27 DWTPs (n=4 or 8) was positive for PMMoV; samples from 10 of these DWTPs were always contaminated. There was a significant difference in the occurrence of PMMoV among geographical regions but not a seasonal difference. PMMoV was frequently detected in samples that were negative for human enteric virus or Escherichia coli. A phylogenetic analysis based on the partial nucleotide sequences of the PMMoV coat protein gene in 12 water samples from 9 DWTPs indicated that there are genetically diverse PMMoV strains present in drinking water sources in Japan. To our knowledge, this is the first study to demonstrate the occurrence of PMMoV in environmental waters across wide geographical regions.
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