ArticleApplied and environmental microbiology2013
Survival of bactericidal antibiotic treatment by a persister subpopulation of Listeria monocytogenes.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Global Transcriptional Differences inAntibiotics (Basel, Switzerland) · 2026Article
- Biocides as drivers of antibiotic resistance: A critical review of environmental implications and public health risks.Environmental science and ecotechnology · 2025Review
- Molecular mechanism and application of emerging technologies in study of bacterial persisters.BMC microbiology · 2024Review
- Multi-species biofilms of environmental microbiota isolated from fruit packing facilities promoted tolerance ofBiofilm · 2024Article
- Discovery of antibiotics that selectively kill metabolically dormant bacteria.Cell chemical biology · 2024Article
- Survival strategies ofFood science and biotechnology · 2023Review
- The Viable But Non-Culturable State ofFrontiers in cellular and infection microbiology · 2022Review
- Cellular Self-Digestion and Persistence in Bacteria.Microorganisms · 2021Review
- Metabolites Potentiate Nitrofurans in Nongrowing Escherichia coli.Antimicrobial agents and chemotherapy · 2021Article
- SigB-regulated antioxidant functions in gram-positive bacteria.World journal of microbiology & biotechnology · 2021Review
- Toxin-antitoxin HicAB regulates the formation of persister cells responsible for the acid stress resistance in Acetobacter pasteurianus.Applied microbiology and biotechnology · 2021Article
- The general stress response ofMicrobiology (Reading, England) · 2020Article
- Predictive biology: modelling, understanding and harnessing microbial complexity.Nature reviews. Microbiology · 2020Review
- A Quantitative Survey of Bacterial Persistence in the Presence of Antibiotics: Towards Antipersister Antimicrobial Discovery.Antibiotics (Basel, Switzerland) · 2020Article
- Review
- Food-Associated Stress Primes Foodborne Pathogens for the Gastrointestinal Phase of Infection.Frontiers in microbiology · 2018Review
- Listeria monocytogenes switches from dissemination to persistence by adopting a vacuolar lifestyle in epithelial cells.PLoS pathogens · 2017Article
- Listeria monocytogenes Biofilms in the Wonderland of Food Industry.Pathogens (Basel, Switzerland) · 2017Review
- Novel RpoS-Dependent Mechanisms Strengthen the Envelope Permeability Barrier during Stationary Phase.Journal of bacteriology · 2017Article
- The Influence of the Toxin/Antitoxin mazEF on Growth and Survival of Listeria monocytogenes under Stress.Toxins · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, which are a dormant antibiotic-tolerant subpopulation. The purpose of this study was to determine whether L. monocytogenes can form persisters and how bacterial physiology affects the number of persisters in the population. A stationary-phase culture of L. monocytogenes was adjusted to 10(8) CFU ml(-1), and 10(3) to 10(4) CFU ml(-1) survived 72-h treatment with 100 μg of norfloxacin ml(-1), indicating a persister subpopulation. This survival was not caused by antibiotic resistance as regrown persisters were as sensitive to norfloxacin as the parental strain. Higher numbers of persisters (10(5) to 10(6)) were surviving when older stationary phase or surface-associated cells were treated with 100 μg of norfloxacin ml(-1). The number of persisters was similar when a ΔsigB mutant and the wild type were treated with norfloxacin, but the killing rate was higher in the ΔsigB mutant. Dormant norfloxacin persisters could be activated by the addition of fermentable carbohydrates and subsequently killed by gentamicin; however, a stable surviving subpopulation of 10(3) CFU ml(-1) remained. Nitrofurantoin that has a growth-independent mode of action was effective against both growing and dormant cells, suggesting that eradication of persisters is possible. Our study adds L. monocytogenes to the list of bacterial species capable of surviving bactericidal antibiotics in a dormant stage, and this persister phenomenon should be borne in mind when developing treatment regimens.
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