ArticleApplied and environmental microbiology2013
Using transcriptomics to improve butanol tolerance of Synechocystis sp. strain PCC 6803.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- Adaptive Laboratory Evolution inMicroorganisms · 2026Review
- Bacterial Tolerance to 1-Butanol and 2-Butanol: Quantitative Assessment and Transcriptomic Response.International journal of molecular sciences · 2024Article
- Using Publicly Available RNA-seq Data for Expression Analysis of Genes of Interest.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803.NPJ systems biology and applications · 2023Article
- Design and construction of microbial cell factories based on systems biology.Synthetic and systems biotechnology · 2023Review
- The Synthesis and Assembly of a Truncated Cyanophage Genome and Its Expression in a Heterogenous Host.Life (Basel, Switzerland) · 2022Article
- Roles of Close Homologues SigB and SigD in Heat and High Light Acclimation of the CyanobacteriumLife (Basel, Switzerland) · 2022Article
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- Expression of HeterologousFrontiers in plant science · 2020Article
- Understanding and engineering alcohol-tolerant bacteria using OMICS technology.World journal of microbiology & biotechnology · 2018Review
- Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1.Journal of bacteriology · 2018Article
- Evaluation of the ethanol tolerance for wild and mutantBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Ethylene causes transcriptomic changes inPlant direct · 2018Article
- CRISPR Gene Perturbations Provide Insights for Improving Bacterial Biofuel Tolerance.Frontiers in bioengineering and biotechnology · 2018Article
- Article
- FabG can function as PhaB for poly-3-hydroxybutyrate biosynthesis in photosynthetic cyanobacteria Synechocystis sp. PCC 6803.Bioengineered · 2017Article
- Identification of alcohol stress tolerance genes ofBiotechnology for biofuels · 2017Article
- Systematic and functional identification of small non-coding RNAs associated with exogenous biofuel stress in cyanobacteriumBiotechnology for biofuels · 2017Article
- Metabolic responses to ethanol and butanol inBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are emerging as promising hosts for production of advanced biofuels such as n-butanol and alkanes. However, cyanobacteria suffer from the same product inhibition problems as those that plague other microbial biofuel hosts. High concentrations of butanol severely reduce growth, and even small amounts can negatively affect metabolic processes. An understanding of how cyanobacteria are affected by their biofuel product can enable identification of engineering strategies for improving their tolerance. Here we used transcriptome sequencing (RNA-Seq) to assess the transcriptome response of Synechocystis sp. strain PCC 6803 to two concentrations of exogenous n-butanol. Approximately 80 transcripts were differentially expressed at 40 mg/liter butanol, and 280 transcripts were different at 1 g/liter butanol. Our results suggest a compromised cell membrane, impaired photosynthetic electron transport, and reduced biosynthesis. Accumulation of intracellular reactive oxygen species (ROS) scaled with butanol concentration. Using the physiology and transcriptomics data, we selected several genes for overexpression in an attempt to improve butanol tolerance. We found that overexpression of several proteins, notably, the small heat shock protein HspA, improved tolerance to butanol. Transcriptomics-guided engineering created more solvent-tolerant cyanobacteria strains that could be the foundation for a more productive biofuel host.
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