ArticleApplied and environmental microbiology2013
Association of a D-alanyl-D-alanine carboxypeptidase gene with the formation of aberrantly shaped cells during the induction of viable but nonculturable Vibrio parahaemolyticus.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Revisiting Strategies for Bacterial Enumeration: The Case for Viable but Nonculturable (VBNC) Cells in Supplement Products.Probiotics and antimicrobial proteins · 2026Review
- The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin.Microbial biotechnology · 2025Article
- Significance of Viable But Non-culturable (VBNC) State in Vibrios and Other Pathogenic Bacteria: Induction, Detection and the Role of Resuscitation Promoting Factors (Rpf).Current microbiology · 2024Review
- Understanding the transition to viable but non-culturable state in Escherichia coli W3110: a comprehensive analysis of potential spectrochemical biomarkers.World journal of microbiology & biotechnology · 2024Article
- Novel expression system based on enhanced permeability of Vibrio natriegens cells induced by D,D- carboxypeptidase overexpression.World journal of microbiology & biotechnology · 2023Article
- Identification of determinants for entering into a viable but nonculturable state in Vibrio alginolyticus by Tn-seq.Applied microbiology and biotechnology · 2023Article
- New Insights into the Mechanism of Antibacterial Action of Synthetic PeptideAntibiotics (Basel, Switzerland) · 2022Article
- Overview of VBNC, a survival strategy for microorganisms.3 Biotech · 2022Review
- Class A Penicillin-Binding Protein C Is Responsible for Stress Response by Regulation of Peptidoglycan Assembly in Clavibacter michiganensis.Microbiology spectrum · 2022Article
- Colistin potentiation in multidrug-resistantFrontiers in microbiology · 2022Article
- Analysis of HubP-dependent cell pole protein targeting in Vibrio cholerae uncovers novel motility regulators.PLoS genetics · 2022Article
- Experimental evolution ofFrontiers in microbiology · 2022Article
- Designing a novel chimeric multi-epitope vaccine againstFrontiers in medicine · 2022Article
- Morphological and physiological changes in Lentilactobacillus hilgardii cells after cold plasma treatment.Scientific reports · 2020Article
- First-time characterization of viable but non-culturable Proteus mirabilis: Induction and resuscitation.Journal of cellular and molecular medicine · 2020Article
- Alterations in the Cell Wall ofFrontiers in microbiology · 2020Article
- Characterization of putative DD-carboxypeptidase-encoding genes in Mycobacterium smegmatis.Scientific reports · 2019Article
- Morphology ofPrzeglad gastroenterologiczny · 2018Review
- Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens.Frontiers in microbiology · 2017Review
- Determinants of Bacterial Morphology: From Fundamentals to Possibilities for Antimicrobial Targeting.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio parahaemolyticus is a halophilic Gram-negative bacterium that causes human gastroenteritis. When the viable but nonculturable (VBNC) state of this bacterium was induced by incubation at 4°C in Morita minimal salt solution containing 0.5% NaCl, the rod-shaped cells became coccoid, and various aberrantly shaped intermediates were formed in the initial stage. This study examined the factors that influence the formation of these aberrantly shaped cells. The proportion of aberrantly shaped cells was not affected in a medium containing D-cycloserine (50 μg/ml) but was lower in a medium containing cephalosporin C (10 μg/ml) than in the control medium without antibiotics. The proportion of aberrantly shaped cells was higher in a culture medium that contained 0.5% NaCl than in culture media containing 1.0 or 1.5% NaCl. The expression of 15 of 17 selected genes associated with cell wall synthesis was enhanced, and the expression of VP2468 (dacB), which encodes D-alanyl-D-alanine carboxypeptidase, was enhanced the most. The proportion of aberrantly shaped cells was significantly lower in the dacB mutant strain than in the parent strain, but the proportion was restored in the presence of the complementary dacB gene. This study suggests that disturbance of the dynamics of cell wall synthesis by enhanced expression of the VP2468 gene is associated with the formation of aberrantly shaped cells in the initial stage of induction of VBNC V. parahaemolyticus cells under specific conditions.
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