ArticleIntegrative biology : quantitative biosciences from nano to macro2013
A serial micropipette microfluidic device with applications to cancer cell repeated deformation studies.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 72 citations in OpenAlex.
- Trade-off movement between hydraulic resistance escape and shear stress escape by cancer cells.Biophysical journal · 2025Article
- Mechanical deformation and death of circulating tumor cells in the bloodstream.Cancer metastasis reviews · 2024Review
- Cytoplasmic viscosity is a potential biomarker for metastatic breast cancer cells.Nanoscale advances · 2024Article
- Phenotypic Heterogeneity, Bidirectionality, Universal Cues, Plasticity, Mechanics, and the Tumor Microenvironment Drive Cancer Metastasis.Biomolecules · 2024Review
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- High-throughput mechanophenotyping of multicellular spheroids using a microfluidic micropipette aspiration chip.Lab on a chip · 2023Article
- Measuring cell deformation by microfluidics.Frontiers in bioengineering and biotechnology · 2023Review
- Biophysical studies of cancer cells' traverse-vessel behaviors under different pressures revealed cells' motion state transition.Scientific reports · 2022Article
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- A mesoscale mechanical model of cellular interactions.Biophysical journal · 2021Article
- Cancer-Nano-Interaction: From Cellular Uptake to Mechanobiological Responses.International journal of molecular sciences · 2021Review
- Biophysical Informatics Approach For Quantifying Phenotypic Heterogeneity In Cancer Cell Migration In Confined Microenvironments.Bioinformatics (Oxford, England) · 2021Article
- A formalism for modelling traction forces and cell shape evolution during cell migration in various biomedical processes.Biomechanics and modeling in mechanobiology · 2021Article
- Non-invasive acquisition of mechanical properties of cells via passive microfluidic mechanisms: A review.Biomicrofluidics · 2021Review
- Morphodynamic signatures of MDA-MB-231 single cells and cell doublets undergoing invasion in confined microenvironments.Scientific reports · 2021Article
- A constriction channel analysis of astrocytoma stiffness and disease progression.Biomicrofluidics · 2021Article
- Review
- Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances.Research and practice in thrombosis and haemostasis · 2020Review
- Traversing behavior of tumor cells in three-dimensional platforms with different topography.PloS one · 2020Article
- Biomaterials to model and measure epithelial cancers.Nature reviews. Materials · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Cells are complex viscoelastic materials that are frequently in deformed morphological states, particularly during the cancer invasion process. The ability to study cell mechanical deformability in an accessible way can be enabling in many areas of research where biomechanics is important, from cancer metastasis to immune response to stem cell differentiation. Furthermore, phenomena in biology are frequently exhibited in high multiplicity. For instance, during metastasis, cells undergoing non-proteolytic invasion squeeze through a multitude of physiological barriers, including many small pores in the dense extracellular matrix (ECM) of the tumor stroma. Therefore, it is important to perform multiple measurements of the same property even for the same cell in order to fully appreciate its dynamics and variability, especially in the high recurrence regime. We have created a simple and minimalistic micropipette system with automated operational procedures that can sample the deformation and relaxation dynamics of single-cells serially and in a parallel manner. We demonstrated its ability to elucidate the impact of an initial cell deformation event on subsequent deformations for untreated and paclitaxel treated MDA-MB-231 metastatic breast cancer cells, and we examined contributions from the cell nucleus during whole-cell micropipette experiments. Finally we developed an empirical model that characterizes the serial factor, which describes the reduction in cost for cell deformations across sequential constrictions. We performed experiments using spatial, temporal, and force scales that match physiological and biomechanical processes, thus potentially enabling a qualitatively more pertinent representation of the functional attributes of cell deformability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.