ArticleInsect biochemistry and molecular biology2013
Tribolium castaneum Transformer-2 regulates sex determination and development in both males and females.
Article in Insect biochemistry and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Review
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- A Conserved Somatic Sex Determination Cascade Instructs Trait-Specific Sexual Dimorphism in Horned Dung Beetles.Evolution & development · 2025Article
- Anatomical changes of Tenebrio molitor and Tribolium castaneum during complete metamorphosis.Cell and tissue research · 2024Article
- scRNA-seq Reveals Novel Genetic Pathways and Sex Chromosome Regulation in Tribolium Spermatogenesis.Genome biology and evolution · 2024Article
- Article
- The Roles ofInsects · 2023Article
- NInsect biochemistry and molecular biology · 2023Article
- When does the female bias arise? Insights from the sex determination cascade of a flea beetle with a strongly skewed sex ratio.Functional & integrative genomics · 2023Article
- Transformer 2β regulates the alternative splicing of cell cycle regulatory genes to promote the malignant phenotype of ovarian cancer.Oncology research · 2023Article
- femaleless Controls Sex Determination and Dosage Compensation Pathways in Females of Anopheles Mosquitoes.Current biology : CB · 2021Article
- Identification and Functional Analysis of the Sex-DeterminerFrontiers in physiology · 2021Article
- Article
- Two of the three Transformer-2 genes are required for ovarian development in Aedes albopictus.Insect biochemistry and molecular biology · 2019Article
- Precise staging of beetle horn formation in Trypoxylus dichotomus reveals the pleiotropic roles of doublesex depending on the spatiotemporal developmental contexts.PLoS genetics · 2019Article
- Article
- Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.PLoS genetics · 2017Article
- Identification and functional analyses of sex determination genes in the sexually dimorphic stag beetle Cyclommatus metallifer.BMC genomics · 2016Article
- Expression analysis of Drosophila doublesex, transformer-2, intersex, fruitless-like, and vitellogenin homologs in the parahaploid predator Metaseiulus occidentalis (Chelicerata: Acari: Phytoseiidae).Experimental & applied acarology · 2015Article
- Article
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2 authors at 1 institution in 1 country.
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Abstract
Tribolium castaneum Transformer (TcTra) is essential for female sex determination and maintenance through the regulation of sex-specific splicing of doublesex (dsx) pre-mRNA. In females, TcTra also regulates the sex-specific splicing of its own pre-mRNA to ensure continuous production of functional Tra protein. Transformer protein is absent in males and hence dsx pre-mRNA is spliced in a default mode. The mechanisms by which males inhibit the production of functional Tra protein are not known. Here, we report on functional characterization of transformer-2 (tra-2) gene (an ortholog of Drosophila transformer-2) in T. castaneum. RNA interference-mediated knockdown in the expression of gene coding for tra-2 in female pupae or adults resulted in the production of male-specific isoform of dsx and both female and male isoforms of tra suggesting that Tra-2 is essential for the female-specific splicing of tra and dsx pre-mRNAs. Interestingly, knockdown of tra-2 in males did not affect the splicing of dsx but resulted in the production of both female and male isoforms of tra suggesting that Tra-2 suppresses female-specific splicing of tra pre-mRNA in males. This dual regulation of sex-specific splicing of tra pre-mRNA ensures a tight regulation of sex determination and maintenance. These data suggest a critical role for Tra-2 in suppression of female sex determination cascade in males. In addition, RNAi studies showed that Tra-2 is also required for successful embryonic and larval development in both sexes.
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