ArticleJournal of bacteriology2013
Aconitase-mediated posttranscriptional regulation of Helicobacter pylori peptidoglycan deacetylase.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Unusual Relationship between Iron Deprivation and Organophosphate Hydrolase Expression.Applied and environmental microbiology · 2023Article
- Gram-Negative Bacterial Envelope Homeostasis under Oxidative and Nitrosative Stress.Microorganisms · 2022Review
- Riboregulation in the Major Gastric PathogenFrontiers in microbiology · 2021Review
- The Rho-Independent Transcription Terminator for theInfection and immunity · 2019Article
- Disruption of the OLE ribonucleoprotein complex causes magnesium toxicity in Bacillus halodurans.Molecular microbiology · 2019Article
- Protein Activity Sensing in Bacteria in Regulating Metabolism and Motility.Frontiers in microbiology · 2019Review
- Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.Molecular microbiology · 2018Article
- Morphology ofPrzeglad gastroenterologiczny · 2018Review
- From bacterial killing to immune modulation: Recent insights into the functions of lysozyme.PLoS pathogens · 2017Review
- Protein signature characterizingInfectious agents and cancer · 2017Article
- Oxidative and nitrosative stress defences of Helicobacter and Campylobacter species that counteract mammalian immunity.FEMS microbiology reviews · 2016Article
- Localisation and protein-protein interactions of the Helicobacter pylori taxis sensor TlpD and their connection to metabolic functions.Scientific reports · 2016Article
- Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori.Journal of bacteriology · 2015Article
- A novel DNA-binding protein plays an important role in Helicobacter pylori stress tolerance and survival in the host.Journal of bacteriology · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some bacterial aconitases are bifunctional proteins that function in the citric acid cycle and act as posttranscriptional regulators in response to iron levels and oxidative stress. We explore the role of aconitase (AcnB) in Helicobacter pylori as a posttranscriptional regulator of the cell wall-modifying enzyme peptidoglycan deacetylase, PgdA. Under oxidative stress, PgdA is highly expressed and confers resistance to lysozyme in wild-type cells. PgdA protein expression as well as transcript abundance is significantly decreased in an acnB mutant. In the wild type, pgdA mRNA half-life was 13 min, whereas the half-life for the acnB strain was 7 min. Based on electrophoretic mobility shift assays and RNA footprinting, the H. pylori apo-AcnB binds to the 3'-untranslated region of the pgdA RNA transcript. Some of the protected bases (from footprinting) were localized in proposed stem-loop structures. AcnB-pgdA transcript binding was abolished by the addition of iron. The acnB strain is more susceptible to lysozyme-mediated killing and was attenuated in its ability to colonize mice. The results support a model whereby apo-AcnB directly interacts with the pgdA transcript to enhance stability and increase deacetylase enzyme expression, which impacts in vivo survival.
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