ArticleJournal of bacteriology2013
Diffusible signal factor (DSF) synthase RpfF of Xylella fastidiosa is a multifunction protein also required for response to DSF.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
- Diffusible Signal Factors andBiology · 2025Review
- Constructing a Novel Disease Resistance Mechanism Model for Cruciferous Crops: An Example From Black Rot.Molecular plant pathology · 2025Review
- BDSF Analogues Inhibit Quorum Sensing-Regulated Biofilm Production inMicroorganisms · 2024Article
- Phosphonates ofInternational journal of molecular sciences · 2024Article
- A Stenotrophomonas maltophilia TetR-Like Transcriptional Regulator Involved in Fatty Acid Metabolism Is Controlled by Quorum Sensing Signals.Applied and environmental microbiology · 2023Article
- Research on Diffusible Signal Factor-Mediated Quorum Sensing inMolecules (Basel, Switzerland) · 2023Review
- Exploring the xylem-sap to unravel biological features ofFrontiers in plant science · 2023Article
- Transcriptomic analysis of chloride tolerance in Leptospirillum ferriphilum DSM 14647 adapted to NaCl.PloS one · 2022Article
- Article
- Review
- A protein that controls the onset of aThe EMBO journal · 2018Article
- A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry.Molecules (Basel, Switzerland) · 2017Article
- Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence.PLoS pathogens · 2017Article
- Bacterial pathogenesis of plants: future challenges from a microbial perspective: Challenges in Bacterial Molecular Plant Pathology.Molecular plant pathology · 2016Review
- The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa.Scientific reports · 2016Article
- Article
- The trans-kingdom identification of negative regulators of pathogen hypervirulence.FEMS microbiology reviews · 2016Review
- Burkholderia Diffusible Signal Factor Signals to Francisella novicida To Disperse Biofilm and Increase Siderophore Production.Applied and environmental microbiology · 2015Article
- The DSF Family of Cell-Cell Signals: An Expanding Class of Bacterial Virulence Regulators.PLoS pathogens · 2015Review
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6 authors.
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Abstract
Xylella fastidiosa, like related Xanthomonas species, employs an Rpf cell-cell communication system consisting of a diffusible signal factor (DSF) synthase, RpfF, and a DSF sensor, RpfC, to coordinate expression of virulence genes. While phenotypes of a ΔrpfF strain in Xanthomonas campestris could be complemented by its own DSF, the DSF produced by X. fastidiosa (XfDSF) did not restore expression of the XfDSF-dependent genes hxfA and hxfB to a ΔrpfF strain of X. fastidiosa, suggesting that RpfF is involved in XfDSF sensing or XfDSF-dependent signaling. To test this conjecture, rpfC and rpfF of X. campestris were replaced by those of X. fastidiosa, and the contribution of each gene to the induction of a X. campestris DSF-dependent gene was assessed. As in X. fastidiosa, XfDSF-dependent signaling required both X. fastidiosa proteins RpfF and RpfC. RpfF repressed RpfC signaling activity, which in turn was derepressed by XfDSF. A mutated X. fastidiosa RpfF protein with two substitutions of glutamate to alanine in its active site was incapable of XfDSF production yet enabled a response to XfDSF, indicating that XfDSF production and the response to XfDSF are two separate functions in which RpfF is involved. This mutant was also hypervirulent to grape, demonstrating the antivirulence effects of XfDSF itself in X. fastidiosa. The Rpf system of X. fastidiosa is thus a novel example of a quorum-sensing signal synthase that is also involved in the response to the signal molecule that it synthesizes.
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