ArticleJournal of bacteriology2013
Membrane chaperone SecDF plays a role in the secretion of Listeria monocytogenes major virulence factors.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- Virulence Potential and Antimicrobial Resistance ofMicroorganisms · 2024Article
- A comprehensive investigation of protein expression profiles inFrontiers in microbiology · 2023Article
- Multi-Omics ofProteomes · 2022Article
- Different Shades ofFrontiers in microbiology · 2021Article
- Article
- Listeriolysin O: from bazooka to Swiss army knife.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Exiguobacterium sp. A1b/GX59 isolated from a patient with community-acquired pneumonia and bacteremia: genomic characterization and literature review.BMC infectious diseases · 2017Review
- L-glutamine Induces Expression of Listeria monocytogenes Virulence Genes.PLoS pathogens · 2017Article
- Strategies Used by Bacteria to Grow in Macrophages.Microbiology spectrum · 2016Review
- The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.Journal of industrial microbiology & biotechnology · 2016Article
- Insulin-Like Growth Factor II (IGF-II) Inhibits IL-1β-Induced Cartilage Matrix Loss and Promotes Cartilage Integrity in Experimental Osteoarthritis.Journal of cellular biochemistry · 2015Article
- A systematic proteomic analysis of Listeria monocytogenes house-keeping protein secretion systems.Molecular & cellular proteomics : MCP · 2014Article
- SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.PloS one · 2014Article
- How Listeria monocytogenes organizes its surface for virulence.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocation system is the primary mediator of this secretion. Here, we validate an important role for SecDF, a component of the Sec system, in the secretion of several critical L. monocytogenes virulence factors. A ΔsecDF mutant is demonstrated to exhibit impaired membrane translocation of listeriolysin O (LLO), PlcA, PlcB, and ActA, factors that mediate L. monocytogenes phagosomal escape and spread from cell to cell. This impaired translocation was monitored by accumulation of the factors on the bacterial membrane and by reduced activity upon secretion. This defect in secretion is shown to be associated with a severe intracellular growth defect of the ΔsecDF mutant in macrophages and a less virulent phenotype in mice, despite normal growth in laboratory medium. We further show that SecDF is upregulated when the bacteria reside in macrophage phagosomes and that it is necessary for efficient phagosomal escape. Taken together, these data support the premise that SecDF plays a role as a chaperone that facilitates the translocation of L. monocytogenes virulence factors during infection.
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