ArticleBiochemical and biophysical research communications2013
Asymmetric packaging of polymerases within vesicular stomatitis virus.
Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Stochastic model of vesicular stomatitis virus replication reveals mutational effects on virion production.PLoS computational biology · 2024Article
- Assembly and transport of filovirus nucleocapsids.PLoS pathogens · 2022Review
- Polymerase-tagged respiratory syncytial virus reveals a dynamic rearrangement of the ribonucleocapsid complex during infection.PLoS pathogens · 2020Article
- Nucleocapsid Structure of Negative Strand RNA Virus.Viruses · 2020Review
- Correlative iPALM and SEM resolves virus cavity and Gag lattice defects in HIV virions.European biophysics journal : EBJ · 2019Article
- Tracking the Fate of Genetically Distinct Vesicular Stomatitis Virus Matrix Proteins Highlights the Role for Late Domains in Assembly.Journal of virology · 2015Article
- A Dynein Light Chain 1 Binding Motif in Rabies Virus Polymerase L Protein Plays a Role in Microtubule Reorganization and Viral Primary Transcription.Journal of virology · 2015Article
- Superresolution imaging of viral protein trafficking.Medical microbiology and immunology · 2015Review
- Vesicular stomatitis virus polymerase's strong affinity to its template suggests exotic transcription models.PLoS computational biology · 2014Article
- Lipids in cell biology: how can we understand them better?Molecular biology of the cell · 2014Article
- Review
- Sample preparation for single virion atomic force microscopy and super-resolution fluorescence imaging.Journal of visualized experiments : JoVE · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Vesicular stomatitis virus (VSV) is a prototypic negative sense single-stranded RNA virus. The bullet-shape appearance of the virion results from tightly wound helical turns of the nucleoprotein encapsidated RNA template (N-RNA) around a central cavity. Transcription and replication require polymerase complexes, which include a catalytic subunit L and a template-binding subunit P. L and P are inferred to be in the cavity, however lacking direct observation, their exact position has remained unclear. Using super-resolution fluorescence imaging and atomic force microscopy (AFM) on single VSV virions, we show that L and P are packaged asymmetrically towards the blunt end of the virus. The number of L and P proteins varies between individual virions and they occupy 57 ± 12 nm of the 150 nm central cavity of the virus. Our finding positions the polymerases at the opposite end of the genome with respect to the only transcriptional promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.