ArticleActa biomaterialia2014
The effect of detergents on the basement membrane complex of a biologic scaffold material.
Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
111 citing papers in PubMed, 211 citations in OpenAlex.
- Co-Electrospinning Extracellular Matrix with Polycaprolactone Enables a Modular Approach to Balance Bioactivity and Mechanics of a Multifunctional Bone Wrap.Advanced healthcare materials · 2026Article
- Natural Biomaterials for Osteochondral Repair: From Source to Strategy.Advanced healthcare materials · 2026Review
- Supercritical COMaterials today. Bio · 2026Article
- Detergent-Based Decellularization Preserves Extracellular Matrix Ultrastructure in Ovine Soft Tissues.Biomimetics (Basel, Switzerland) · 2026Article
- Experimental Analysis and Modeling Study of Impedance Changes in Decellularized and Recellularized Peripheral Nerves.Bioengineering (Basel, Switzerland) · 2026Article
- AlloDerm Versus Cortiva: A Multicenter Pooled Analysis of Clinical and Economic Outcomes in Breast Reconstruction.Plastic and reconstructive surgery. Global open · 2026Article
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Rapid SDS/trypsin decellularization of rat submandibular gland yields an ECM scaffold supporting salivary gland tissue engineering.Frontiers in bioengineering and biotechnology · 2026Article
- Low-detergent sonication decellularization preserves extracellular matrix architecture and enhances cell infiltration in human acellular dermal matrix.Frontiers in bioengineering and biotechnology · 2026Article
- From Production to the Clinic: Decellularized Extracellular Matrix as a Biomaterial for Tissue Engineering and Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2025Review
- Dentinogenic Effect of BMP-7 on Wharton's Jelly Mesenchymal Stem Cells Cultured in Decellularized Dental Pulp.International journal of molecular sciences · 2025Article
- Systematic analysis of the influence of enzymatic and chemical detergents on structure, biomechanics and biocompatibility of decellularized vascular grafts.Journal of materials science. Materials in medicine · 2025Article
- Decellularized lymph node sections with preserved extracellular matrix for stromal cell culture.Scientific reports · 2025Article
- The Role and Involvement of Functional Three-Dimensional Porcine-Derived Collagen Matrix Biomaterials in Periodontal Regeneration: A Comprehensive Review.Journal of functional biomaterials · 2025Review
- Article
- Development of UROGRAFT: A Bladder Acellular Matrix-Based Composite for Advanced Cystoplasty, Highlighting the Role of Graft Shape and Composition.ACS biomaterials science & engineering · 2025Article
- Scaffold implantationAnnals of surgical treatment and research · 2025Article
- Decellularized Cell-Secreted Extracellular Matrices as Biomaterials for Tissue Engineering.Small science · 2025Review
- Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices.Bioengineering (Basel, Switzerland) · 2025Review
- Decellularization techniques: unveiling the blueprint for tracheal tissue engineering.Frontiers in bioengineering and biotechnology · 2025Review
51 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The basement membrane complex (BMC) is a critical component of the extracellular matrix (ECM) that supports and facilitates the growth of cells. This study investigates four detergents commonly used in the process of tissue decellularization and their effect upon the BMC. The BMC of porcine urinary bladder was subjected to 3% Triton-X 100, 8mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), 4% sodium deoxycholate or 1% sodium dodecyl sulfate (SDS) for 24h. The BMC structure for each treatment group was assessed by immunolabeling, scanning electron microscopy (SEM) and second harmonic generation (SHG) imaging of the fiber network. The composition was assessed by quantification of dsDNA, glycosaminoglycans (GAG) and collagen content. The results showed that collagen fibers within samples treated with 1% SDS and 8mM CHAPS were denatured, and the ECM contained fewer GAG compared with samples treated with 3% Triton X-100 or 4% sodium deoxycholate. Human microvascular endothelial cells (HMEC) were seeded onto each BMC and cultured for 7 days. Cell-ECM interactions were investigated by immunolabeling for integrin β-1, SEM imaging and semi-quantitative assessment of cellular infiltration, phenotype and confluence. HMEC cultured on a BMC treated with 3% Triton X-100 were more confluent and had a normal phenotype compared with HMEC cultured on a BMC treated with 4% sodium deoxycholate, 8mM CHAPS and 1% SDS. Both 8mM CHAPS and 1% SDS damaged the BMC to the extent that seeded HMEC were able to infiltrate the damaged sub-basement membrane tissue, showed decreased confluence and an atypical phenotype. The choice of detergents used for tissue decellularization can have a marked effect upon the integrity of the BMC of the resultant bioscaffold.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.