ArticleMicrobial cell factories2013
Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 140 citations in OpenAlex.
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- Challenges in elucidating ethylene glycol metabolism in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Investigation of Bottleneck Enzyme Through Flux Balance Analysis to Improve Glycolic Acid Production in Escherichia coli.Journal of microbiology (Seoul, Korea) · 2024Article
- Exploring yeast biodiversity and process conditions for optimizing ethylene glycol conversion into glycolic acid.FEMS yeast research · 2024Article
- Profiling proteomic responses to hexokinase-II depletion in terpene-producingEngineering microbiology · 2023Article
- Inducible Synthetic Growth Regulation Using the ClpXP Proteasome Enhances cis,cis-Muconic Acid and Glycolic Acid Yields inACS synthetic biology · 2023Article
- Repurposing of waste PET by microbial biotransformation to functionalized materials for additive manufacturing.Journal of industrial microbiology & biotechnology · 2023Article
- Green Hydrogel Synthesis: Emphasis on Proteomics and Polymer Particle-Protein Interaction.Polymers · 2022Review
- Engineering the glyoxylate cycle for chemical bioproduction.Frontiers in bioengineering and biotechnology · 2022Review
- EngineeringFrontiers in bioengineering and biotechnology · 2022Article
- Towards Sustainable Oxalic Acid from COChemSusChem · 2021Review
- Model-guided development of an evolutionarily stable yeast chassis.Molecular systems biology · 2021Article
- Biosensor-Based Multigene Pathway Optimization for Enhancing the Production of Glycolate.Applied and environmental microbiology · 2021Article
- Exploring the oxygenase function of Form II Rubisco for production of glycolate from COAMB Express · 2021Article
- Hydroquinone exposure accumulates neutral lipid by the activation of CDP-DAG pathway inToxicology research · 2021Article
- CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate.Biotechnology for biofuels · 2021Article
- Engineering Escherichia coli for the utilization of ethylene glycol.Microbial cell factories · 2021Article
- Engineering microbial pathways for production of bio-based chemicals from lignocellulosic sugars: current status and perspectives.Biotechnology for biofuels · 2020Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlycolic acid is a C2 hydroxy acid that is a widely used chemical compound. It can be polymerised to produce biodegradable polymers with excellent gas barrier properties. Currently, glycolic acid is produced in a chemical process using fossil resources and toxic chemicals. Biotechnological production of glycolic acid using renewable resources is a desirable alternative.
resultsThe yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are suitable organisms for glycolic acid production since they are acid tolerant and can grow in the presence of up to 50 g l(-1) glycolic acid. We engineered S. cerevisiae and K. lactis for glycolic acid production using the reactions of the glyoxylate cycle to produce glyoxylic acid and then reducing it to glycolic acid. The expression of a high affinity glyoxylate reductase alone already led to glycolic acid production. The production was further improved by deleting genes encoding malate synthase and the cytosolic form of isocitrate dehydrogenase. The engineered S. cerevisiae strain produced up to about 1 g l(-1) of glycolic acid in a medium containing d-xylose and ethanol. Similar modifications in K. lactis resulted in a much higher glycolic acid titer. In a bioreactor cultivation with D-xylose and ethanol up to 15 g l(-1) of glycolic acid was obtained.
conclusionsThis is the first demonstration of engineering yeast to produce glycolic acid. Prior to this work glycolic acid production through the glyoxylate cycle has only been reported in bacteria. The benefit of a yeast host is the possibility for glycolic acid production also at low pH, which was demonstrated in flask cultivations. Production of glycolic acid was first shown in S. cerevisiae. To test whether a Crabtree negative yeast would be better suited for glycolic acid production we engineered K. lactis in the same way and demonstrated it to be a better host for glycolic acid production.
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