ArticleStem cell reports2013
Reprogramming to pluripotency using designer TALE transcription factors targeting enhancers.
Article in Stem cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 78 citations in OpenAlex.
- Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing.Cell genomics · 2025Article
- Development of artificial transcription factors and their applications in cell reprograming, genetic screen, and disease treatment.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Review
- Activation of the imprinted Prader-Willi Syndrome locus by CRISPR-based epigenome editing.bioRxiv : the preprint server for biology · 2024Article
- Review
- Genome Editing and Cardiac Regeneration.Advances in experimental medicine and biology · 2023Article
- Human iPSCs and Genome Editing Technologies for Precision Cardiovascular Tissue Engineering.Frontiers in cell and developmental biology · 2021Review
- Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs.Cell stem cell · 2019Article
- Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming.Stem cell reports · 2019Article
- The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells.Nature communications · 2019Article
- Rejuvenation of β cells by epigenetic editing.The Journal of clinical investigation · 2019Article
- Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays.The CRISPR journal · 2018Article
- Reprogramming cell fate with artificial transcription factors.FEBS letters · 2018Review
- Genome Editing Redefines Precision Medicine in the Cardiovascular Field.Stem cells international · 2018Review
- Mammalian Transcription Factor Networks: Recent Advances in Interrogating Biological Complexity.Cell systems · 2017Review
- CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.Nature biotechnology · 2017Article
- Advanced Gene Manipulation Methods for Stem Cell Theranostics.Theranostics · 2017Review
- Article
- Reprogramming cell fate with a genome-scale library of artificial transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- To CRISPR and beyond: the evolution of genome editing in stem cells.Regenerative medicine · 2016Review
- CRISPR-Mediated Epigenome Editing.The Yale journal of biology and medicine · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The modular DNA recognition code of the transcription-activator-like effectors (TALEs) from plant pathogenic bacterial genus Xanthomonas provides a powerful genetic tool to create designer transcription factors (dTFs) targeting specific DNA sequences for manipulating gene expression. Previous studies have suggested critical roles of enhancers in gene regulation and reprogramming. Here, we report dTF activator targeting the distal enhancer of the Pou5f1 (Oct4) locus induces epigenetic changes, reactivates its expression, and substitutes exogenous OCT4 in reprogramming mouse embryonic fibroblast cells (MEFs) to induced pluripotent stem cells (iPSCs). Similarly, dTF activator targeting a Nanog enhancer activates Nanog expression and reprograms epiblast stem cells (EpiSCs) to iPSCs. Conversely, dTF repressors targeting the same genetic elements inhibit expression of these loci, and effectively block reprogramming. This study indicates that dTFs targeting specific enhancers can be used to study other biological processes such as transdifferentiation or directed differentiation of stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.