ArticleStem cell reports2013
Enhanced hemangioblast generation and improved vascular repair and regeneration from embryonic stem cells by defined transcription factors.
Article in Stem cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- ETV2/ER71 regulates hematovascular lineage generation and vascularization through an H3K9 demethylase, KDM4A.iScience · 2025Article
- FGF primes angioblast formation by inducing ETV2 and LMO2 via FGFR1/BRAF/MEK/ERK.Cellular and molecular life sciences : CMLS · 2021Article
- From Stripes to a Beating Heart: Early Cardiac Development in Zebrafish.Journal of cardiovascular development and disease · 2021Review
- A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells.Stem cell reports · 2021Article
- GATA2 Promotes Hematopoietic Development and Represses Cardiac Differentiation of Human Mesoderm.Stem cell reports · 2019Article
- HOXB4 Promotes Hemogenic Endothelium Formation without Perturbing Endothelial Cell Development.Stem cell reports · 2018Article
- Cellular and Molecular Heterogeneity Associated with Vessel Formation Processes.BioMed research international · 2018Review
- Etv2 as an essential regulator of mesodermal lineage development.Cardiovascular research · 2017Review
- Hemovascular Progenitors in the Kidney Require Sphingosine-1-Phosphate Receptor 1 for Vascular Development.Journal of the American Society of Nephrology : JASN · 2016Article
- The Hemogenic Competence of Endothelial Progenitors Is Restricted by Runx1 Silencing during Embryonic Development.Cell reports · 2016Article
- Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation.Developmental cell · 2016Article
- Injury-Mediated Vascular Regeneration Requires Endothelial ER71/ETV2.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- The ETS Factor, ETV2: a Master Regulator for Vascular Endothelial Cell Development.Molecules and cells · 2015Review
- LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Lost in translation: pluripotent stem cell-derived hematopoiesis.EMBO molecular medicine · 2015Review
- Induction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71/ETV2.EMBO reports · 2015Article
- An expandable, inducible hemangioblast state regulated by fibroblast growth factor.Stem cell reports · 2014Article
- Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators.Nature communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
The fetal liver kinase 1 (FLK-1)(+) hemangioblast can generate hematopoietic, endothelial, and smooth muscle cells (SMCs). ER71/ETV2, GATA2, and SCL form a core transcriptional network in hemangioblast development. Transient coexpression of these three factors during mesoderm formation stage in mouse embryonic stem cells (ESCs) robustly enhanced hemangioblast generation by activating bone morphogenetic protein (BMP) and FLK-1 signaling while inhibiting phosphatidylinositol 3-kinase, WNT signaling, and cardiac output. Moreover, etsrp, gata2, and scl inhibition converted hematopoietic field of the zebrafish anterior lateral plate mesoderm to cardiac. FLK-1(+) hemangioblasts generated by transient coexpression of the three factors (ER71-GATA2-SCL [EGS]-induced FLK-1(+)) effectively produced hematopoietic, endothelial, and SMCs in culture and in vivo. Importantly, EGS-induced FLK-1(+) hemangioblasts, when codelivered with mesenchymal stem cells as spheroids, were protected from apoptosis and generated functional endothelial cells and SMCs in ischemic mouse hindlimbs, resulting in improved blood perfusion and limb salvage. ESC-derived, EGS-induced FLK-1(+) hemangioblasts could provide an attractive cell source for future hematopoietic and vascular repair and regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.