ArticleHawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health2013
Rapid multiplex PCR assay to identify respiratory viral pathogens: moving forward diagnosing the common cold.
Article in Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2018Pooled it
- Diagnosis of Respiratory Infections Using Syndromic Panels: A Ct-Based Approach Beyond Qualitative Detection.Microorganisms · 2026Article
- Distribution of Viral Respiratory Infections during the COVID-19 Pandemic Using the FilmArray Respiratory Panel.Biomedicines · 2022Article
- Evaluation of viral respiratory pathogens in children aged under five hospitalized with lower respiratory tract infections.Northern clinics of Istanbul · 2022Article
- Advances in laboratory assays for detecting human metapneumovirus.Annals of translational medicine · 2020Review
- Rapid respiratory panel testing: Impact of active antimicrobial stewardship.American journal of infection control · 2019Article
- Observational
- Comparison of Respiratory Pathogen Detection in Upper versus Lower Respiratory Tract Samples Using the BioFire FilmArray Respiratory Panel in the Immunocompromised Host.Canadian respiratory journal · 2018Article
- FilmArray Respiratory Panel Assay: Comparison of Nasopharyngeal Swabs and Bronchoalveolar Lavage Samples.Journal of clinical microbiology · 2015Article
- Management of respiratory viral infections in hematopoietic cell transplant recipients and patients with hematologic malignancies.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Review
- Management of lower respiratory tract infection in outpatient settings: Focus on clarithromycin.Lung India : official organ of Indian Chest SocietyReview
- Prevalence and Predictors for Respiratory Viral Infections among Liver Disease Patients.Euroasian journal of hepato-gastroenterologyArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upper respiratory tract infections (URIs) can be a serious burden to the healthcare system. The majority of URIs are viral in etiology, but definitive diagnosis can prove difficult due to frequently overlapping clinical presentations of viral and bacterial infections, and the variable sensitivity, and lengthy turn-around time of viral culture. We tested new automated nested multiplex PCR technology, the FilmArray(®) system, in the TAMC department of clinical investigations, to determine the feasibility of replacing the standard viral culture with a rapid turn-around system. We conducted a feasibility study using a single-blinded comparison study, comparing PCR results with archived viral culture results from a convenience sample of cryopreserved archived nasopharyngeal swabs from acutely ill ED patients who presented with complaints of URI symptoms. A total of 61 archived samples were processed. Viral culture had previously identified 31 positive specimens from these samples. The automated nested multiplex PCR detected 38 positive samples. In total, PCR was 94.5% concordant with the previously positive viral culture results. However, PCR was only 63.4% concordant with the negative viral culture results, owing to PCR detection of 11 additional viral pathogens not recovered on viral culture. The average time to process a sample was 75 minutes. We determined that an automated nested multiplex PCR is a feasible alternative to viral culture in an acute clinical setting. We were able to detect at least 94.5% as many viral pathogens as viral culture is able to identify, with a faster turn-around time.
Indexed as
Identifiers
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Registered trials
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