ArticleJournal of bone and mineral metabolism2014
Phosphate enhances reactive oxygen species production and suppresses osteoblastic differentiation.
Article in Journal of bone and mineral metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
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- Lycium-Barbarum Polysaccharide-Loaded Dual-Crosslinked Rigid Hydrogel Enhances Bone Healing in Diabetic Bone Defects by Scavenging Reactive Oxygen Species.Advanced healthcare materials · 2025Article
- Impact of Exposure to Leaves From Metal-Polluted Sites on the Developmental Parameters of Larvae of the Dark Sword-Grass, Agrotis ipsilon (Lepidoptera: Noctuidae).Archives of environmental contamination and toxicology · 2024Article
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- Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair.Bioactive materials · 2023Article
- 8-Nitro-cGMP suppresses mineralization by mouse osteoblasts.Journal of clinical biochemistry and nutrition · 2022Article
- Extracellular Inorganic Phosphate-Induced Release of Reactive Oxygen Species: Roles in Physiological Processes and Disease Development.International journal of molecular sciences · 2021Review
- Stage-, sex- and tissue-related changes in HEcotoxicology (London, England) · 2021Article
- Reactive oxygen species (ROS) generation as an underlying mechanism of inorganic phosphate (PFree radical biology & medicine · 2020Article
- Antioxidant enzyme activity in responses to environmentally induced oxidative stress in the 5th instar nymphs of Aiolopus thalassinus (Orthoptera: Acrididae).Environmental science and pollution research international · 2019Article
- MicroRNA profiling analysis of developing berries for 'Kyoho' and its early-ripening mutant during berry ripening.BMC plant biology · 2018Article
- Small molecules modified biomimetic gelatin/hydroxyapatite nanofibers constructing an ideal osteogenic microenvironment with significantly enhanced cranial bone formation.International journal of nanomedicine · 2018Article
- Evaluation of oxidative stress biomarkers in Aiolopus thalassinus (Orthoptera: Acrididae) collected from areas polluted by the fertilizer industry.Ecotoxicology (London, England) · 2017Article
- Inhibition of GSK-3β increases trabecular bone volume but not cortical bone volume in adenine-induced uremic mice with severe hyperparathyroidism.Physiological reports · 2016Article
- Inorganic Phosphate Activates the AKT/mTORC1 Pathway and Shortens the Life Span of an α‑Klotho-Deficient Model.Journal of the American Society of Nephrology : JASN · 2016Article
- Phosphate enhances Fgf23 expression through reactive oxygen species in UMR-106 cells.Journal of bone and mineral metabolism · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphate has been shown to work as a signaling molecule in several cells including endothelial cells and chondrocytes. However, it is largely unknown how phosphate affects osteoblastic cells. In the present study, we investigated the effects of phosphate on reactive oxygen species (ROS) production and osteoblastic differentiation in murine osteoblastic MC3T3-E1 cells. Phosphate increased production of ROS in MC3T3-E1 cells and the inhibitors of sodium-phosphate cotransporter and NADPH oxidase suppressed ROS production by phosphate. Silencing Nox1 and Nox4 also inhibited the increase of ROS by phosphate. Phosphate also decreased alkaline phosphatase activity induced by bone morphogenetic protein 2 and this inhibition was abrogated by an inhibitor of NADPH oxidase. Furthermore, phosphate decreased the expression of osteoblastic marker genes in MC3T3-E1 cells. These results indicate that phosphate suppresses osteoblastic differentiation at least in part by enhancing ROS production in MC3T3-E1 cells.
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