ArticleInternational journal of legal medicine2014
Body fluid identification by integrated analysis of DNA methylation and body fluid-specific microbial DNA.
Article in International journal of legal medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 100 citations in OpenAlex.
- DNA methylation and epigenomic profiling post-mortem of human remain analogs (Sus scrofa) in southern Ontario, Canada.Journal of forensic sciences · 2026Article
- Article
- Application of a new composite genetic marker semen-specific methylation-microhaplotype in the analysis of semen-vaginal fluid mixtures.Royal Society open science · 2025Article
- Forensic Microbiology: When, Where and How.Microorganisms · 2024Review
- Development of a novel panel for blood identification based on blood-specific CpG-linked SNP markers.International journal of legal medicine · 2024Article
- Rapid and visual detection of specific bacteria for saliva and vaginal fluid identification with the lateral flow dipstick strategy.International journal of legal medicine · 2023Article
- A Review on Microbial Species for Forensic Body Fluid Identification in Healthy and Diseased Humans.Current microbiology · 2023Review
- Article
- Application of Microbiome in Forensics.Genomics, proteomics & bioinformatics · 2023Review
- Establishment of a co-analysis system for personal identification and body fluid identification: a preliminary report.International journal of legal medicine · 2022Article
- Design and optimization of a 16S microbial qPCR multiplex for the presumptive identification of feces, saliva, vaginal and menstrual secretions.Journal of forensic sciences · 2022Article
- On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime.Genes · 2021Review
- Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid.Diagnostics (Basel, Switzerland) · 2020Review
- Detection and discrimination of seminal fluid using attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy combined with chemometrics.International journal of legal medicine · 2020Article
- Characterization of DNA methylation-based markers for human body fluid identification in forensics: a critical review.International journal of legal medicine · 2020Review
- Microbial community dissimilarity for source tracking with application in forensic studies.PloS one · 2020Article
- Forensic discrimination of menstrual blood and peripheral blood using attenuated total reflectance (ATR)-Fourier transform infrared (FT-IR) spectroscopy and chemometrics.International journal of legal medicine · 2020Article
- Characterization of tissue-specific biomarkers with the expression of circRNAs in forensically relevant body fluids.International journal of legal medicine · 2019Article
- Specific microbes of saliva and vaginal fluid of Guangdong Han females based on 16S rDNA high-throughput sequencing.International journal of legal medicine · 2019Article
- Microbial forensics: new breakthroughs and future prospects.Applied microbiology and biotechnology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of body fluids found at crime scenes provides important information that can support a link between sample donors and actual criminal acts. Previous studies have reported that DNA methylation analysis at several tissue-specific differentially methylated regions (tDMRs) enables successful identification of semen, and the detection of certain bacterial DNA can allow for identification of saliva and vaginal fluid. In the present study, a method for detecting bacterial DNA was integrated into a previously reported multiplex methylation-sensitive restriction enzyme-polymerase chain reaction. The developed multiplex PCR was modified by the addition of a new semen-specific marker and by including amplicons for the 16S ribosomal RNA gene of saliva- and vaginal fluid-specific bacteria to improve the efficacy to detect a specific type of body fluid. Using the developed multiplex system, semen was distinguishable by unmethylation at the USP49, DACT1, and PFN3 tDMRs and by hypermethylation at L81528, and saliva could be identified by detection of saliva-specific bacteria, Veillonella atypica and/or Streptococcus salivarius. Additionally, vaginal fluid and menstrual blood were differentiated from other body fluids by hypomethylation at the PFN3 tDMR and the presence of vaginal fluid-specific bacteria, Lactobacillus crispatus and/or Lactobacillus gasseri. Because the developed multiplex system uses the same biological source of DNA for individual identification profiling and simultaneously analyses various types of body fluid in one PCR reaction, this method will facilitate more efficient body fluid identification in forensic casework.
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