ArticleThe Journal of cell biology1990
In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 66 citations in OpenAlex.
- Viscoelastic Relaxation of the Nuclear Envelope Does Not Cause the Collapse of the Spindle After Ablation inJournal of undergraduate reports in physics · 2021Article
- Electron tomography reveals aspects of spindle structure important for mechanical stability at metaphase.Molecular biology of the cell · 2020Article
- Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles.European biophysics journal : EBJ · 2018Review
- A Brief History of Research on Mitotic Mechanisms.Biology · 2016Review
- Pivoting of microtubules around the spindle pole accelerates kinetochore capture.Nature cell biology · 2013Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Cytoplasmic microtubule organization in fission yeast.Yeast (Chichester, England) · 2006Review
- The roles of fission yeast ase1 in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signaling.Molecular biology of the cell · 2005Article
- Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts.Molecular biology of the cell · 2000Article
- Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4.Molecular biology of the cell · 1998Article
- Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function.Molecular biology of the cell · 1996Article
- DSK1, a novel kinesin-related protein from the diatom Cylindrotheca fusiformis that is involved in anaphase spindle elongation.The Journal of cell biology · 1996Article
- Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.The Journal of cell biology · 1995Article
- The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability.The Journal of cell biology · 1995Article
- Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation.The Journal of cell biology · 1995Article
- Human gamma-tubulin functions in fission yeast.The Journal of cell biology · 1994Article
- Interpolar spindle microtubules in PTK cells.The Journal of cell biology · 1993Article
- Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans.The Journal of cell biology · 1993Article
- Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the mechanisms of spindle elongation and chromosome separation in the fission yeast Schizosaccharomyces pombe, we have developed an in vitro assay using a temperature-sensitive mutant strain, nuc2. At the restrictive temperature, nuc2 cells are arrested at a metaphase-like stage with short spindles and condensed chromosomes. After permeabilization of spheroplasts of the arrested cells, spindle elongation was reactivated by addition of ATP and neurotubulin both at the restrictive and the permissive temperatures, but chromosome separation was not. This suggests that the nuc2 cells are impaired in function at a stage before sister chromatid disjunction. Spindle elongation required both ATP and exogenous tubulin and was inhibited by adenylyl imidodiphosphate (AMPPNP) or vanadate. The ends of yeast half-spindle microtubules pulse-labeled with biotinylated tubulin moved past each other during spindle elongation and a gap formed between the original half-spindles. These results suggest that the primary mechanochemical event responsible for spindle elongation is the sliding apart of antiparallel microtubules of the two half-spindles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.