ArticleBiomedical optics express2013
Real-time optical pH measurement in a standard microfluidic cell culture system.
Article in Biomedical optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- Enabling Rapid pH Equilibration Through Acoustic Microstreaming for Efficient pH Regulation in Microliter-Scale Samples with Screen-Printed Electrodes.Biosensors · 2026Article
- Transducer Systems Integrated into Organ-on-a-Chip Devices: From Detection to Fabrication.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Real-Time, Continuous Monitoring of Tissue Chips as an Emerging Opportunity for Biosensing.Sensors (Basel, Switzerland) · 2025Review
- Mammalian cell growth characterisation by a non-invasive plate reader assay.Nature communications · 2024Article
- Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalentsBiomaterials and biosystems · 2022Article
- A Portable 'Plug-and-Play' Fibre Optic Sensor for In-Situ Measurements of pH Values for Microfluidic Applications.Micromachines · 2022Article
- Defect Density-Dependent pH Response of Graphene Derivatives: Towards the Development of pH-Sensitive Graphene Oxide Devices.Nanomaterials (Basel, Switzerland) · 2022Article
- Genetically Encoded Ratiometric pH Sensors for the Measurement of Intra- and Extracellular pH and Internalization Rates.Biosensors · 2022Article
- Review
- PHAIR: a biosensor for pH measurement in air-liquid interface cell culture.Scientific reports · 2021Article
- Visualization and Measurements of Blood Cells Flowing in Microfluidic Systems and Blood Rheology: A Personalized Medicine Perspective.Journal of personalized medicine · 2020Review
- Novel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy.Engineering in life sciences · 2017Article
- A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices.Biomicrofluidics · 2016Article
- Monitoring microbial metabolites using an inductively coupled resonance circuit.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid growth of microfluidic cell culturing in biological and biomedical research and industry calls for fast, non-invasive and reliable methods of evaluating conditions such as pH inside a microfluidic system. We show that by careful calibration it is possible to measure pH within microfluidic chambers with high accuracy and precision, using a direct single-pass measurement of light absorption in a commercially available phenol-red-containing cell culture medium. The measurement is carried out using a standard laboratory microscope and, contrary to previously reported methods, requires no modification of the microfluidic device design. We demonstrate the validity of this method by measuring absorption of light transmitted through 30-micrometer thick microfluidic chambers, using an inverted microscope fitted with a scientific-grade digital camera and two bandpass filters. In the pH range of 7-8, our measurements have a standard deviation and absolute error below 0.05 for a measurement volume smaller than 4 nL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.