ArticleNucleic acids research2014
Natural reassignment of CUU and CUA sense codons to alanine in Ashbya mitochondria.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Regulation of Riboflavin Biosynthesis in Microorganisms and Construction of the Advanced Overproducers of This Vitamin.International journal of molecular sciences · 2025Review
- Mitochondrial genome diversity across the subphylum Saccharomycotina.Frontiers in microbiology · 2023Article
- Mitochondrial genome annotation with MFannot: a critical analysis of gene identification and gene model prediction.Frontiers in plant science · 2023Article
- Refactored genetic codes enable bidirectional genetic isolation.Science (New York, N.Y.) · 2022Article
- Evolutionary Adjustment of tRNA Identity Rules in Bacillariophyta for Recognition by an Aminoacyl-tRNA Synthetase Adds a Facet to the Origin of Diatoms.Journal of molecular evolution · 2022Article
- Naturally Occurring tRNAs With Non-canonical Structures.Frontiers in microbiology · 2020Review
- Evolution and Unprecedented Variants of the Mitochondrial Genetic Code in a Lineage of Green Algae.Genome biology and evolution · 2019Article
- Rapid Genetic Code Evolution in Green Algal Mitochondrial Genomes.Molecular biology and evolution · 2019Article
- Mitogenomics Reveals a Novel Genetic Code in Hemichordata.Genome biology and evolution · 2019Article
- Rewriting the Genetic Code.Annual review of microbiology · 2017Review
- Review
- How tRNAs dictate nuclear codon reassignments: Only a few can capture non-cognate codons.RNA biology · 2017Review
- A novel nuclear genetic code alteration in yeasts and the evolution of codon reassignment in eukaryotes.Genome research · 2016Article
- Non-Standard Genetic Codes Define New Concepts for Protein Engineering.Life (Basel, Switzerland) · 2015Review
- Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology.Nature reviews. Microbiology · 2015Review
- Pathways of Genetic Code Evolution in Ancient and Modern Organisms.Journal of molecular evolution · 2015Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The discovery of diverse codon reassignment events has demonstrated that the canonical genetic code is not universal. Studying coding reassignment at the molecular level is critical for understanding genetic code evolution, and provides clues to genetic code manipulation in synthetic biology. Here we report a novel reassignment event in the mitochondria of Ashbya (Eremothecium) gossypii, a filamentous-growing plant pathogen related to yeast (Saccharomycetaceae). Bioinformatics studies of conserved positions in mitochondrial DNA-encoded proteins suggest that CUU and CUA codons correspond to alanine in A. gossypii, instead of leucine in the standard code or threonine in yeast mitochondria. Reassignment of CUA to Ala was confirmed at the protein level by mass spectrometry. We further demonstrate that a predicted tRNA(Ala)UAG is transcribed and accurately processed in vivo, and is responsible for Ala reassignment. Enzymatic studies reveal that tRNA(Ala)UAG is efficiently recognized by A. gossypii mitochondrial alanyl-tRNA synthetase (AgAlaRS). AlaRS typically recognizes the G3:U70 base pair of tRNA(Ala); a G3A change in Ashbya tRNA(Ala)UAG abolishes its recognition by AgAlaRS. Conversely, an A3G mutation in Saccharomyces cerevisiae tRNA(Thr)UAG confers tRNA recognition by AgAlaRS. Our work highlights the dynamic feature of natural genetic codes in mitochondria, and the relative simplicity by which tRNA identity may be switched.
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