ArticleJournal of the American Society for Mass Spectrometry2013
Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion.
Article in Journal of the American Society for Mass Spectrometry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- High-Throughput Analysis of Protein Adsorption to a Large Library of Polymers Using Liquid Extraction Surface Analysis-Tandem Mass Spectrometry (LESA-MS/MS).Analytical chemistry · 2025Article
- Identifying Process Differences with ToF-SIMS: An MVA Decomposition Strategy.Journal of the American Society for Mass Spectrometry · 2024Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.Advanced healthcare materials · 2021Article
- Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling.Nature communications · 2020Article
- Unsupervised machine learning for exploratory data analysis in imaging mass spectrometry.Mass spectrometry reviews · 2020Review
- Controlling Experimental Parameters to Improve Characterization of Biomaterial Fouling.Frontiers in chemistry · 2020Review
- Dynamic secretome of bone marrow-derived stromal cells reveals a cardioprotective biochemical cocktail.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions.Rapid communications in mass spectrometry : RCM · 2018Article
- High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques.Analytical chemistry · 2016Review
- High-Resolution Ambient MS Imaging of Negative Ions in Positive Ion Mode: Using Dicationic Reagents with the Single-Probe.Journal of the American Society for Mass Spectrometry · 2016Article
- Profiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS.Analytical and bioanalytical chemistry · 2015Article
- High Resolution Tissue Imaging Using the Single-probe Mass Spectrometry under Ambient Conditions.Journal of the American Society for Mass Spectrometry · 2015Article
- Top-down and bottom-up identification of proteins by liquid extraction surface analysis mass spectrometry of healthy and diseased human liver tissue.Journal of the American Society for Mass Spectrometry · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection and identification of proteins adsorbed onto biomaterial surfaces under ambient conditions has significant experimental advantages but has proven to be difficult to achieve with conventional measuring technologies. In this study, we present an adaptation of desorption electrospray ionization (DESI) and liquid extraction surface analysis (LESA) mass spectrometry (MS) coupled with in-situ surface tryptic digestion to identify protein species from a biomaterial surface. Cytochrome c, myoglobin, and BSA in a combination of single and mixture spots were printed in an array format onto Permanox slides, followed by in-situ surface digestion and detection via MS. Automated tandem MS performed on surface peptides was able to identify the proteins via MASCOT. Limits of detection were determined for DESI-MS and a comparison of DESI and LESA-MS peptide spectra characteristics and sensitivity was made. DESI-MS images of the arrays were produced and analyzed with imaging multivariate analysis to automatically separate peptide peaks for each of the proteins within a mixture into distinct components. This is the first time that DESI and LESA-MS have been used for the in-situ detection of surface digested proteins on biomaterial surfaces and presents a promising proof of concept for the use of ambient MS in the rapid and automated analysis of surface proteins.
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