ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Transgene expression in target-defined neuron populations mediated by retrograde infection with adeno-associated viral vectors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
76 citing papers in PubMed, 120 citations in OpenAlex.
- Target-dependent routing of sensory information in the olfactory system.Communications biology · 2026Article
- Evidence that interglomerular inhibition generates non-monotonic concentration-response relationships in mitral/tufted glomeruli in the mouse olfactory bulb.The Journal of physiology · 2026Article
- Rostral Ventromedial Medulla (RVM) Projects to the Lateral Hypothalamic Area (LHA) to Drive Aversion and Anxiety.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Dynamic changes in chloride homeostasis coordinate midbrain inhibitory network activity during reward learning.Nature communications · 2025Article
- Hippocampal neural circuit mechanisms in Alzheimer's disease revealed by viral-genetic circuit mapping.Neurobiology of disease · 2025Review
- A novel GABAergic population in the medial vestibular nucleus maintains wakefulness and gates rapid eye movement sleep.iScience · 2024Article
- The cerebellum directly modulates the substantia nigra dopaminergic activity.Nature neuroscience · 2024Article
- Anatomical topology of extrahippocampal projections from dorsoventral CA pyramidal neurons in mice.Frontiers in neuroanatomy · 2024Article
- Olfactory deficits in aging and Alzheimer's-spotlight on inhibitory interneurons.Frontiers in neuroscience · 2024Review
- Chronic alcohol exposure alters action control via hyperactive premotor corticostriatal activity.Cell reports · 2023Article
- Lighting Up Neural Circuits by Viral Tracing.Neuroscience bulletin · 2022Review
- Neuronal circuitry for recognition memory of object and place in rodent models.Neuroscience and biobehavioral reviews · 2022Review
- Modulating the Excitability of Olfactory Output Neurons Affects Whole-Body Metabolism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions.Communications biology · 2022Article
- A novel H129-based anterograde monosynaptic tracer exhibits features of strong labeling intensity, high tracing efficiency, and reduced retrograde labeling.Molecular neurodegeneration · 2022Article
- Novel Cerebello-Amygdala Connections Provide Missing Link Between Cerebellum and Limbic System.Frontiers in systems neuroscience · 2022Article
- Advances in Gene Delivery Methods to Label and Modulate Activity of Upper Motor Neurons: Implications for Amyotrophic Lateral Sclerosis.Brain sciences · 2021Review
- Gene delivery to the hypoglossal motor system: preclinical studies and translational potential.Gene therapy · 2021Review
- Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Animal Models of Drug Relapse and Craving after Voluntary Abstinence: A Review.Pharmacological reviews · 2021Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Tools enabling the manipulation of well defined neuronal subpopulations are critical for probing complex neuronal networks. Cre recombinase (Cre) mouse driver lines in combination with the Cre-dependent expression of proteins using viral vectors--in particular, recombinant adeno-associated viral vectors (rAAVs)--have emerged as a widely used platform for achieving transgene expression in specified neural populations. However, the ability of rAAVs to further specify neuronal subsets on the basis of their anatomical connectivity has been reported as limited or inconsistent. Here, we systematically tested a variety of widely used neurotropic rAAVs for their ability to mediate retrograde gene transduction in the mouse brain. We tested pseudotyped rAAVs of several common serotypes (rAAV 2/1, 2/5, and 2/9) as well as constructs both with and without Cre-dependent expression switches. Many of the rAAVs tested--in particular, though not exclusively, Cre-dependent vectors--showed a robust capacity for retrograde infection and transgene expression. Retrograde expression was successful over distances as large as 6 mm and in multiple neuron types, including olfactory projection neurons, neocortical pyramidal cells projecting to distinct targets, and corticofugal and modulatory projection neurons. Retrograde infection using transgenes such as ChR2 allowed for optical control or optically assisted electrophysiological identification of neurons defined genetically as well as by their projection target. These results establish a widely accessible tool for achieving combinatorial specificity and stable, long-term transgene expression to isolate precisely defined neuron populations in the intact animal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.