ArticleAmerican journal of physiology. Cell physiology2013
Pharmacological characterization of the P2 receptors profile in the podocytes of the freshly isolated rat glomeruli.
Article in American journal of physiology. Cell physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Renal Implications of Kappa Opioid Receptor Signaling in Sprague-Dawley Rats.Function (Oxford, England) · 2025Article
- PAR1-mediated Non-periodical Synchronized Calcium Oscillations in Human Mesangial Cells.Function (Oxford, England) · 2024Article
- Renin-angiotensin system-mediated nitric oxide signaling in podocytes.American journal of physiology. Renal physiology · 2024Article
- Calcium signalling and transport in the kidney.Nature reviews. Nephrology · 2024Review
- Ion channels and channelopathies in glomeruli.Physiological reviews · 2023Review
- Modulation of P2XPhysiological reports · 2022Article
- Characterization of purinergic receptor 2 signaling in podocytes from diabetic kidneys.iScience · 2021Article
- Role of opioid signaling in kidney damage during the development of salt-induced hypertension.Life science alliance · 2020Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Role of TRPC6 in Progression of Diabetic Kidney Disease.Current hypertension reports · 2019Review
- Characterization of purinergic receptor expression in ARPKD cystic epithelia.Purinergic signalling · 2018Article
- Nitric oxide production by glomerular podocytes.Nitric oxide : biology and chemistry · 2018Article
- Pharmacological inhibition of focal segmental glomerulosclerosis-related, gain of function mutants of TRPC6 channels by semi-synthetic derivatives of larixol.British journal of pharmacology · 2017Article
- Natriuretic Peptide Receptor Guanylyl Cyclase-A in Podocytes is Renoprotective but Dispensable for Physiologic Renal Function.Journal of the American Society of Nephrology : JASN · 2017Article
- Klotho May Ameliorate Proteinuria by Targeting TRPC6 Channels in Podocytes.Journal of the American Society of Nephrology : JASN · 2017Article
- Protease-activated receptors in kidney disease progression.American journal of physiology. Renal physiology · 2016Review
- Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation.PloS one · 2016Article
- Synthesis of an azido-tagged low affinity ratiometric calcium sensor.Tetrahedron · 2015Article
- Podocyte injury in diabetic nephropathy: implications of angiotensin II-dependent activation of TRPC channels.Scientific reports · 2015Article
- TRPC6 channel as an emerging determinant of the podocyte injury susceptibility in kidney diseases.American journal of physiology. Renal physiology · 2015Review
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4 authors at 1 institution in 1 country.
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Abstract
Calcium flux in the podocytes is critical for normal and pathophysiological regulation of these types of cells, and excessive calcium signaling results in podocytes damage and improper glomeruli function. Purinergic activation of P2 receptors is a powerful and rapid signaling process; however, the exact physiological identity of P2 receptors subtypes in podocytes remains essentially unknown. The goal of this study was to determine the P2 receptor profile in podocytes of the intact Sprague-Dawley rat glomeruli using available pharmacological tools. Glomeruli were isolated by differential sieving and loaded with Fluo-4/Fura Red cell permeable calcium indicators, and the purinergic response in the podocytes was analyzed with ratiometric confocal fluorescence measurements. Various P2 receptors activators were tested and compared with the effect of ATP, specifically, UDP, MRS 2365, bzATP, αβ-methylene, 2-meSADP, MRS 4062, and MRS 2768, were analyzed. Antagonists (MRS 2500, 5-BDBD, A438079, and NF 449) were tested when 10 μM ATP was applied as the EC50 for ATP activation of the calcium influx in the podocytes was determined to be 10.7 ± 1.5 μM. Several agonists including MRS 2365 and 2-meSADP caused calcium flux. Importantly, only the P2Y1-specific antagonist MRS 2500 (1 nM) precluded the effects of ATP concentrations of the physiological range. Immunohistochemical analysis confirmed that P2Y1 receptors are highly expressed in the podocytes. We conclude that P2Y1 receptor signaling is the predominant P2Y purinergic pathway in the glomeruli podocytes and P2Y1 might be involved in the pathogenesis of glomerular injury and could be a target for treatment of kidney diseases.
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