ArticleDevelopment (Cambridge, England)2013
Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Highly efficient transgenesis mediated by Tip100 transposon system in medaka.Transgenic research · 2025Article
- Cloning-Free Targeting of Endogenous Loci to Generate Fluorescent Reporters in Medaka.Bio-protocol · 2025Article
- Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase.Biology open · 2024Article
- Article
- Article
- phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.Genetics · 2022Article
- Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.Cell reports · 2021Article
- Article
- Instantaneous isotropic volumetric imaging of fast biological processes.Nature methods · 2019Article
- A Collection of Transgenic Medaka Strains for Efficient Site-Directed Transgenesis Mediated by phiC31 Integrase.G3 (Bethesda, Md.) · 2018Article
- PhiC31-based Site-Specific Transgenesis System for Production of Transgenic Bovine Embryos by Somatic Cell Nuclear Transfer and Intracytoplasmic Sperm Injection.Cell journal · 2018Article
- TGFβ-facilitated optic fissure fusion and the role of bone morphogenetic protein antagonism.Open biology · 2018Article
- Unlinking the methylome pattern from nucleotide sequence, revealed by large-scale in vivo genome engineering and methylome editing in medaka fish.PLoS genetics · 2017Article
- Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model.Journal of visualized experiments : JoVE · 2017Article
- MEPD: medaka expression pattern database, genes and more.Nucleic acids research · 2016Article
- A new model army: Emerging fish models to study the genomics of vertebrate Evo-Devo.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2015Review
- Functional assessment of disease-associated regulatory variants in vivo using a versatile dual colour transgenesis strategy in zebrafish.PLoS genetics · 2015Article
- Review
- Targeted transgene integration overcomes variability of position effects in zebrafish.Development (Cambridge, England) · 2014Article
- Harnessing mobile genetic elements to explore gene regulation.Mobile genetic elements · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Established transgenesis methods for fish model systems allow efficient genomic integration of transgenes. However, thus far a way of controlling copy number and integration sites has not been available, leading to variable transgene expression caused by position effects. The integration of transgenes at predefined genomic positions enables the direct comparison of different transgenes, thereby improving time and cost efficiency. Here, we report an efficient PhiC31-based site-specific transgenesis system for medaka. This system includes features that allow the pre-selection of successfully targeted integrations early on in the injected generation. Pre-selected embryos transmit the correctly integrated transgene through the germline with high efficiency. The landing site design enables a variety of applications, such as reporter and enhancer switch, in addition to the integration of any insert. Importantly, this allows assaying of enhancer activity in a site-specific manner without requiring germline transmission, thus speeding up large-scale analyses of regulatory elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.