ArticleJournal of clinical microbiology2013
Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Occurrence of viral and bacterial pathogens in South American camelids in representative German flocks.Frontiers in veterinary science · 2026Article
- Colloidal silver against macrophage infections and biofilms of atypical mycobacteria.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Article
- Genomic epidemiology ofFrontiers in genetics · 2023Article
- Mycobacterium avium subsp. paratuberculosis exploits miRNA expression to modulate lipid metabolism and macrophage polarisation pathways during infection.Scientific reports · 2022Article
- Optimization of the composition of a solid culture medium for Mycobacterium avium subsp. paratuberculosis using factorial design and response surface methodology.Journal of applied microbiology · 2022Article
- Correlates of vaccine protection againstFrontiers in veterinary science · 2022Article
- Molecular characterisation of Mycobacterium avium subsp. paratuberculosis in Australia.BMC microbiology · 2021Article
- Bio-typing of Mycobacterium avium subspecies paratuberculosis isolates recovered from the Himalayan sheep and goats.Tropical animal health and production · 2021Article
- Comparative Genomics ofFrontiers in veterinary science · 2021Article
- Detection of Wood Mice (Microorganisms · 2020Article
- Influence of Colostrum and Vitamins A, DVeterinary sciences · 2019Article
- The humoral immune response is essential for successful vaccine protection against paratuberculosis in sheep.BMC veterinary research · 2019Article
- Gene expression profiles during subclinical Mycobacterium avium subspecies paratuberculosis infection in sheep can predict disease outcome.Scientific reports · 2019Article
- Complete Genome Sequence of the Telford Type S Strain of Mycobacterium avium subsp. paratuberculosis.Microbiology resource announcements · 2019Article
- Determining an optimal pool size for testing beef herds for Johne's disease in Australia.PloS one · 2019Article
- Flock sensitivity and specificity of pooled fecal qPCR and pooled serum ELISA for screening ovine paratuberculosis.PloS one · 2019Article
- Variation in susceptibility of different breeds of sheep to Mycobacterium avium subspecies paratuberculosis following experimental inoculation.Veterinary research · 2017Article
- An intra-laboratory cultural and real-time PCR method comparison and evaluation for the detection of subclinical paratuberculosis in dairy herds.Folia microbiologica · 2017Article
- Article
- Culture-Independent Identification ofFrontiers in veterinary science · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid culture of Mycobacterium avium subsp. paratuberculosis from clinical samples, such as feces, is the most sensitive antemortem test for the diagnosis of Johne's disease in ruminants. In Australia, New Zealand, the United States, and some other countries, the Bactec 460 system with modified Bactec 12B medium (Becton, Dickinson) has been the most commonly used liquid culture system, but it was discontinued in 2012. In this study, a new liquid culture medium, M7H9C, was developed. It consists of a Middlebrook 7H9 medium base with added Casitone, albumin, dextrose, catalase, egg yolk, mycobactin J, and a cocktail of antibiotics. We found that polyoxyethylene stearate (POES) was not essential for the cultivation of M. avium subsp. paratuberculosis in either the Bactec 12B or the M7H9C medium. The limit of detection determined using pure cultures of the C and S strains of M. avium subsp. paratuberculosis was 7 bacilli per 50 μl inoculum in the two media. The new medium was validated using 784 fecal and tissue samples from sheep and cattle, >25% of which contained viable M. avium subsp. paratuberculosis. Discrepant results for the clinical samples between the two media were mostly associated with samples that contained <10 viable bacilli per gram, but these results were relatively uncommon, and the performances of the two media were not significantly different. M7H9C medium was less than half the cost of the Bactec 12B medium and did not require regular examination during incubation, but a confirmatory IS900 PCR test had to be performed on every culture after the predetermined incubation period.
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