ArticleJournal of clinical microbiology2013
Simultaneous detection and differentiation of human rhino- and enteroviruses in clinical specimens by real-time PCR with locked nucleic Acid probes.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Short- and long-term efficacy of prednisolone for first acute rhinovirus-induced wheezing episode.The Journal of allergy and clinical immunology · 2015Trial
- Rhinovirus Species in Acute Respiratory Tract Infections and the Risk of Asthma in Children.Pediatric pulmonology · 2026Article
- Sensitive detection and differentiation of rhinoviruses and enteroviruses by a nested real-time RT-PCR assay.Microbiology spectrum · 2026Article
- Surveillance of Acute Flaccid Paralysis (AFP) in Greece: 2008-2024.Pathogens (Basel, Switzerland) · 2025Article
- Beyond respiratory syncytial virus and rhinovirus: The other viral respiratory bandits.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2025Review
- Diagnostic potential of rhinovirus C using reverse-transcription loop-mediated isothermal amplification (RT-LAMP).International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Viral acute respiratory illnesses in elite athletes: A 12-month controlled follow-up study.PloS one · 2025Article
- Common Seasonal Pathogens and Epidemiology of Henoch-Schönlein Purpura Among Children.JAMA network open · 2024Article
- Fulminant myocarditis associated with human rhinovirus A66 infection: a case report.Frontiers in pediatrics · 2024Article
- Enhancing pathogen description and antibiotic regimen selection in community-acquired pneumonia through RT-qPCR assays.Frontiers in microbiology · 2024Article
- Human bocavirus 1 coinfection is associated with decreased cytokine expression in the rhinovirus-induced first wheezing episode in children.Clinical and translational allergy · 2023Article
- Enterovirus D68 Outbreak in Children, Finland, August-September 2022.Emerging infectious diseases · 2023Article
- Non-SARS-CoV-2 Respiratory Viruses in Athletes at Major Winter Sport Events, 2021 and 2022.Emerging infectious diseases · 2022Article
- An unusual location of hand, foot and mouth disease.Journal of ultrasound · 2022Article
- Cytokine expression in rhinovirus- vs. respiratory syncytial virus-induced first wheezing episode and its relation to clinical course.Frontiers in immunology · 2022Article
- Increased risk of respiratory viral infections in elite athletes: A controlled study.PloS one · 2021Observational
- Detection of Influenza and Other Respiratory Viruses in Air Sampled From a University Campus: A Longitudinal Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
- Common cold in Team Finland during 2018 Winter Olympic Games (PyeongChang): epidemiology, diagnosis including molecular point-of-care testing (POCT) and treatment.British journal of sports medicine · 2019Observational
- A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus.Diagnostic microbiology and infectious disease · 2019Article
- Characteristics of Hospitalized Rhinovirus-Associated Community-Acquired Pneumonia in Children, Finland, 2003-2014.Frontiers in medicine · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human rhinoviruses (HRVs) and human enteroviruses (HEVs) are significant respiratory pathogens. While HRV infections are restricted to the respiratory tract, HEV infections may spread to secondary target organs. The method of choice for sensitive specific detection of these viruses is reverse transcription (RT)-PCR with primers targeting the conserved 5' noncoding region of the viral RNA. On the other hand, sequence similarities between HRVs and HEVs complicate their differential detection. In this study, we describe the use of locked nucleic acid (LNA) analogues in short double-dye probes which contained only two selectively HRV- or HEV-specific bases. The double-stranded DNA dye BOXTO (4-[6-(benzoxazole-2-yl-(3-methyl-)-2,3-dihydro-(benzo-1,3-thiazole)-2-methylidene)]-1-methyl-quinolinium chloride) was used with the LNA probes in a tricolor real-time PCR assay to allow specific detection of HRVs (probes labeled with 6-carboxyfluorescein [FAM] [green]) and HEVs (Cy5 [red]) with additional melting curve analysis (BOXTO [yellow]). The functionality of the probes was validated in PCR and RT-PCR assays using plasmids containing viral cDNA, quantified viral RNA transcripts, cultivated rhino- and enterovirus prototypes, and clinical specimens. Of 100 HRV and 63 HEV prototypes, the probes correctly identified all HEVs except one that produced only a BOXTO signal. Among 118 clinical specimens with sequencing results, concordant results were obtained for 116 specimens. Two specimens were reactive with both probes, but sequencing yielded only a single virus. Real-time PCR with LNA probes allowed sensitive group-specific identification of HRVs and HEVs and would enable relative copy number determination. The assay is suitable for rapid and accurate differential detection of HRVs and HEVs in a diagnostic laboratory setting.
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