ArticleScience translational medicine2013
A host-based RT-PCR gene expression signature to identify acute respiratory viral infection.
Article in Science translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 144 citations in OpenAlex.
- Minimal transmission in an influenza A (H3N2) human challenge-transmission model within a controlled exposure environment.PLoS pathogens · 2020Trial
- Development of an objective gene expression panel as an alternative to self-reported symptom scores in human influenza challenge trials.Journal of translational medicine · 2017Trial
- A novel host-proteome signature for distinguishing between acute bacterial and viral infections.PloS one · 2015Trial
- Article
- A four-gene signature from blood to exclude bacterial etiology of lower respiratory tract infection in adults.Nature communications · 2025Article
- Molecular dynamics of the host response to Streptococcus pneumoniae pneumonia in baboons.Animal models and experimental medicine · 2025Article
- Direct single cell-type gene expression analysis in peripheral blood: novel ratio-based gene expression biomarkers using 2 novel monocyte reference genes (PSAP and CTSS) for detection of bacterial infection.Human molecular genetics · 2025Article
- Approach to Short Duration Fever in Children in Office Practice.Indian journal of pediatrics · 2025Review
- A Four-Gene Signature from Blood to Exclude Bacterial Etiology of Lower Respiratory Tract Infection in Adults.Research square · 2025Article
- SARS-CoV-2 human challenge reveals biomarkers that discriminate early and late phases of respiratory viral infections.Nature communications · 2024Article
- Review: Diagnostic Potential for Collaborative Pharyngitis Biomarkers.The Journal of infectious diseases · 2024Review
- Application of a Machine Learning-Based Classification Approach for Developing Host Protein Diagnostic Models for Infectious Disease.Diagnostics (Basel, Switzerland) · 2024Article
- Implementation of a Prospective Index-Cluster Sampling Strategy for the Detection of Presymptomatic Viral Respiratory Infection in Undergraduate Students.Open forum infectious diseases · 2024Article
- Priorities and Progress in Diagnostic Research by the Antibacterial Resistance Leadership Group.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Review
- An automated DNA computing platform for rapid etiological diagnostics.Science advances · 2022Article
- Systematic comparison of published host gene expression signatures for bacterial/viral discrimination.Genome medicine · 2022Article
- Host Transcriptional Signatures Predict Etiology in Community-Acquired Pneumonia: Potential Antibiotic Stewardship Tools.Biomarker insights · 2022Article
- Comparing the Diagnostic Accuracy of Clinician Judgment to a Novel Host Response Diagnostic for Acute Respiratory Illness.Open forum infectious diseases · 2021Article
- Discriminating Bacterial and Viral Infection Using a Rapid Host Gene Expression Test.Critical care medicine · 2021Article
- Blood transcriptional biomarkers of acute viral infection for detection of pre-symptomatic SARS-CoV-2 infection: a nested, case-control diagnostic accuracy study.The Lancet. Microbe · 2021Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 1 country.
Funding
Abstract
Improved ways to diagnose acute respiratory viral infections could decrease inappropriate antibacterial use and serve as a vital triage mechanism in the event of a potential viral pandemic. Measurement of the host response to infection is an alternative to pathogen-based diagnostic testing and may improve diagnostic accuracy. We have developed a host-based assay with a reverse transcription polymerase chain reaction (RT-PCR) TaqMan low-density array (TLDA) platform for classifying respiratory viral infection. We developed the assay using two cohorts experimentally infected with influenza A H3N2/Wisconsin or influenza A H1N1/Brisbane, and validated the assay in a sample of adults presenting to the emergency department with fever (n = 102) and in healthy volunteers (n = 41). Peripheral blood RNA samples were obtained from individuals who underwent experimental viral challenge or who presented to the emergency department and had microbiologically proven viral respiratory infection or systemic bacterial infection. The selected gene set on the RT-PCR TLDA assay classified participants with experimentally induced influenza H3N2 and H1N1 infection with 100 and 87% accuracy, respectively. We validated this host gene expression signature in a cohort of 102 individuals arriving at the emergency department. The sensitivity of the RT-PCR test was 89% [95% confidence interval (CI), 72 to 98%], and the specificity was 94% (95% CI, 86 to 99%). These results show that RT-PCR-based detection of a host gene expression signature can classify individuals with respiratory viral infection and sets the stage for prospective evaluation of this diagnostic approach in a clinical setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.