ArticleBiophysical journal2013
Measuring the lamellarity of giant lipid vesicles with differential interference contrast microscopy.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Protocell formation on micrometeorites.Scientific reports · 2026Article
- Understanding the Mechanisms of Supported Lipid Membranes Reshaping into Tubular Networks Using Quantitative DIC Microscopy.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Identifying and Manipulating Giant Vesicles: Review of Recent Approaches.Micromachines · 2022Review
- Quantitative Label-Free Imaging of Lipid Domains in Single Bilayers by Hyperspectral Coherent Raman Scattering.Analytical chemistry · 2020Article
- Lipid Bilayer Thickness Measured by Quantitative DIC Reveals Phase Transitions and Effects of Substrate Hydrophilicity.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- In vitro characterization of sonothrombolysis and echocontrast agents to treat ischemic stroke.Scientific reports · 2019Article
- Glycan-decorated protocells: novel features for rebuilding cellular processes.Interface focus · 2019Review
- Quantitative imaging of lipid droplets in single cells.The Analyst · 2019Review
- Modulating Thiazole Orange Aggregation in Giant Lipid Vesicles: Photophysical Study Associated with FLIM and FCS.ACS omega · 2017Article
- Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.European biophysics journal : EBJ · 2017Review
- Quantitative imaging of lipids in live mouse oocytes and early embryos using CARS microscopy.Development (Cambridge, England) · 2016Article
- Effects of lipid composition and solution conditions on the mechanical properties of membrane vesicles.Membranes · 2015Article
- Article
- Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds.Nature nanotechnology · 2014Article
- Quantitative analysis of the lamellarity of giant liposomes prepared by the inverted emulsion method.Biophysical journal · 2014Article
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Authors and funding
4 authors.
Funding
Abstract
Giant unilamellar vesicles are a widely utilized model membrane system, providing free-standing bilayers unaffected by support-induced artifacts. To measure the lamellarity of such vesicles, fluorescence microscopy is one commonly utilized technique, but it has the inherent disadvantages of requiring lipid staining, thereby affecting the intrinsic physical and chemical properties of the vesicles, and it requires a calibration by statistical analysis of a vesicle ensemble. Herein we present what we believe to be a novel label-free optical method to determine the lamellarity of giant vesicles based on quantitative differential interference contrast (qDIC) microscopy. The method is validated by comparison with fluorescence microscopy on a statistically significant number of vesicles, showing correlated quantization of the lamellarity. Importantly, qDIC requires neither sample-dependent calibration nor sample staining, and thus can measure the lamellarity of any giant vesicle without additional preparation or interference with subsequent investigations. Furthermore, qDIC requires only a microscope equipped with differential interference contrast and a digital camera.
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