ArticleJournal of cell science2013
A role for the Golgi matrix protein giantin in ciliogenesis through control of the localization of dynein-2.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to previously unrecognized intermediate stages of early ciliogenesis.Nature communications · 2026Article
- Structure and tethering mechanism of dynein-2 intermediate chains in intraflagellar transport.The EMBO journal · 2024Article
- The factory, the antenna and the scaffold: the three-way interplay between the Golgi, cilium and extracellular matrix underlying tissue function.Biology open · 2023Review
- Ciliogenesis membrane dynamics and organization.Seminars in cell & developmental biology · 2023Review
- Pedigree-based study to identify GOLGB1 as a risk gene for bipolar disorder.Translational psychiatry · 2022Article
- Giantin is required for intracellular N-terminal processing of type I procollagen.The Journal of cell biology · 2021Article
- WDR34, a candidate gene for non-syndromic rod-cone dystrophy.Clinical genetics · 2021Article
- Recent advances in understanding assembly of the primary cilium membrane.Faculty reviews · 2021Review
- A Biomarker Panel of Radiation-Upregulated miRNA as Signature for Ionizing Radiation Exposure.Life (Basel, Switzerland) · 2020Article
- Article
- HDAC3 and HDAC8 are required for cilia assembly and elongation.Biology open · 2019Article
- Ciliary and cytoskeletal functions of an ancient monooxygenase essential for bioactive amidated peptide synthesis.Cellular and molecular life sciences : CMLS · 2019Review
- US3 Kinase-Mediated Phosphorylation of Tegument Protein VP8 Plays a Critical Role in the Cellular Localization of VP8 and Its Effect on the Lipid Metabolism of Bovine Herpesvirus 1-Infected Cells.Journal of virology · 2019Article
- Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells.Biomolecules · 2018Article
- Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.eLife · 2018Article
- Emerging mechanisms of dynein transport in the cytoplasm versus the cilium.Biochemical Society transactions · 2018Review
- Actin-dependent regulation of cilia length by the inverted formin FHDC1.Molecular biology of the cell · 2018Article
- Regulator of calcineurin-2 is a centriolar protein with a role in cilia length control.Journal of cell science · 2018Article
- Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression.Journal of cell science · 2017Article
- The Golgi matrix protein giantin is required for normal cilia function in zebrafish.Biology open · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
The correct formation of primary cilia is central to the development and function of nearly all cells and tissues. Cilia grow from the mother centriole by extension of a microtubule core, the axoneme, which is then surrounded with a specialized ciliary membrane that is continuous with the plasma membrane. Intraflagellar transport moves particles along the length of the axoneme to direct assembly of the cilium and is also required for proper cilia function. The microtubule motor, cytoplasmic dynein-2 mediates retrograde transport along the axoneme from the tip to the base; dynein-2 is also required for some aspects of cilia formation. In most cells, the Golgi lies adjacent to the centrioles and key components of the cilia machinery localize to this organelle. Golgi-localized proteins have also been implicated in ciliogenesis and in intraflagellar transport. Here, we show that the transmembrane Golgi matrix protein giantin (GOLGB1) is required for ciliogenesis. We show that giantin is not required for the Rab11-Rabin8-Rab8 pathway that has been implicated in the early stages of ciliary membrane formation. Instead we find that suppression of giantin results in mis-localization of WDR34, the intermediate chain of dynein-2. Highly effective depletion of giantin or WDR34 leads to an inability of cells to form primary cilia. Partial depletion of giantin or of WDR34 leads to an increase in cilia length consistent with the concept that giantin acts through dynein-2. Our data implicate giantin in ciliogenesis through control of dynein-2 localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.