ArticleThe Journal of biological chemistry2013
The ferredoxin:NAD+ oxidoreductase (Rnf) from the acetogen Acetobacterium woodii requires Na+ and is reversibly coupled to the membrane potential.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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83 citing papers in PubMed, 157 citations in OpenAlex.
- The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea.Applied microbiology and biotechnology · 2025Review
- Flavin transferase ApbE: From discovery to applications.The Journal of biological chemistry · 2025Review
- Molecular principles of redox-coupled sodium pumping of the ancient Rnf machinery.Nature communications · 2025Article
- Acetate Shock Loads Enhance CO Uptake Rates of Anaerobic Microbiomes.Microbial biotechnology · 2024Article
- Multi-scale reactor designs extend the physical limits ofbioRxiv : the preprint server for biology · 2024Article
- Harnessing acetogenic bacteria for one-carbon valorization toward sustainable chemical production.RSC chemical biology · 2024Review
- Architecture of the RNF1 complex that drives biological nitrogen fixation.Nature chemical biology · 2024Article
- Redirecting electron flow in Acetobacterium woodii enables growth on CO and improves growth on formate.Nature communications · 2024Article
- Evolutionary obstacles and not C-F bond strength make PFAS persistent.Microbial biotechnology · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- Current status of carbon monoxide dehydrogenases (CODH) and their potential for electrochemical applications.Bioresources and bioprocessing · 2023Review
- A new metabolic trait in an acetogen: Mixed acid fermentation of fructose in a methylene-tetrahydrofolate reductase mutant of Acetobacterium woodii.Environmental microbiology reports · 2023Article
- Lactate formation from fructose or C1 compounds in the acetogen Acetobacterium woodii by metabolic engineering.Applied microbiology and biotechnology · 2023Article
- Reprogramming the metabolism of an acetogenic bacterium to homoformatogenesis.The ISME journal · 2023Article
- Mechanisms for Generating Low Potential Electrons across the Metabolic Diversity of Nitrogen-Fixing Bacteria.Applied and environmental microbiology · 2023Review
- Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium.Frontiers in microbiology · 2023Article
- Purification and structural characterization of the NaNature communications · 2022Article
- Rnf and Fix Have Specific Roles during Aerobic Nitrogen Fixation in Azotobacter vinelandii.Applied and environmental microbiology · 2022Article
- A Third Way of Energy Conservation in Acetogenic Bacteria.Microbiology spectrum · 2022Article
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23 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
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Abstract
The anaerobic acetogenic bacterium Acetobacterium woodii has a novel Na(+)-translocating electron transport chain that couples electron transfer from reduced ferredoxin to NAD(+) with the generation of a primary electrochemical Na(+) potential across its cytoplasmic membrane. In previous assays in which Ti(3+) was used to reduce ferredoxin, Na(+) transport was observed, but not a Na(+) dependence of the electron transfer reaction. Here, we describe a new biological reduction system for ferredoxin in which ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase from A. woodii. Using CO-reduced ferredoxin, NAD(+) reduction was highly specific and strictly dependent on ferredoxin and occurred at a rate of 50 milliunits/mg of protein. Most important, this assay revealed for the first time a strict Na(+) dependence of this electron transfer reaction. The Km was 0.2 mm. Na(+) could be partly substituted by Li(+). Na(+) dependence was observed at neutral and acidic pH values, indicating the exclusive use of Na(+) as a coupling ion. Electron transport from reduced ferredoxin to NAD(+) was coupled to electrogenic Na(+) transport, indicating the generation of ΔμNa(+). Vice versa, endergonic ferredoxin reduction with NADH as reductant was possible, but only in the presence of ΔμNa(+), and was accompanied by Na(+) efflux out of the vesicles. This is consistent with the hypothesis that Rnf also catalyzes ferredoxin reduction at the expense of an electrochemical Na(+) gradient. The physiological significance of this finding is discussed.
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