ArticleBMC microbiology2013
Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- The Effects of Glucosamine and Chondroitin Sulfate on Gut Microbial Composition: A Systematic Review of Evidence from Animal and Human Studies.Nutrients · 2019Pooled it
- Microbial modulation of proteoglycan and glycosaminoglycan biosynthesis in a three-dimensional corneal epithelium model.Frontiers in cellular and infection microbiology · 2026Article
- Recombinant MAM from Faecalibacterium duncaniae exhibits a protective effect in DNBS-induced colitis.Microbial cell factories · 2025Article
- Pathogens that infect mammalian cells via sulfonated glycosaminoglycans.Frontiers in cellular and infection microbiology · 2025Review
- Prodrug-conjugated tumor-seeking commensals for targeted cancer therapy.Nature communications · 2024Article
- The regulation of simulated artificial oro-gastrointestinal transit stress on the adhesion of Lactobacillus plantarum S7.Microbial cell factories · 2023Article
- Urinary Glycosaminoglycans Are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.ACS infectious diseases · 2023Article
- Crystal Structures ofJournal of applied glycoscience · 2023Article
- Donor-dependent fecal microbiota transplantation efficacy against necrotizing enterocolitis in preterm pigs.NPJ biofilms and microbiomes · 2022Article
- Cell Surface Glycosaminoglycans as Receptors for Adhesion ofPolish journal of microbiology · 2022Article
- Lactobacilli Strain Mixture Alleviates Bacterial Vaginosis through Antibacterial and Antagonistic Activity inMicroorganisms · 2022Article
- A Semi-Quantitative Assay to Measure Glycosaminoglycan Degradation by the Urinary Microbiota.Frontiers in cellular and infection microbiology · 2021Article
- Review
- Adherence ofFrontiers in immunology · 2019Article
- The Microbiotic Highway to Health-New Perspective on Food Structure, Gut Microbiota, and Host Inflammation.Nutrients · 2018Review
- Probiotics in human gut microbiota can degrade host glycosaminoglycans.Scientific reports · 2018Article
- Genetic Signatures of DairyFrontiers in microbiology · 2018Article
- Article
- Surface Proteoglycans as Mediators in Bacterial Pathogens Infections.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe adhesion of lactobacilli to the vaginal surface is of paramount importance to develop their probiotic functions. For this reason, the role of HeLa cell surface proteoglycans in the attachment of Lactobacillus salivarius Lv72, a mutualistic strain of vaginal origin, was investigated.
resultsIncubation of cultures with a variety of glycosaminoglycans (chondroitin sulfate A and C, heparin and heparan sulfate) resulted in marked binding interference. However, no single glycosaminoglycan was able to completely abolish cell binding, the sum of all having an additive effect that suggests cooperation between them and recognition of specific adhesins on the bacterial surface. In contrast, chondroitin sulfate B enhanced cell to cell attachment, showing the relevance of the stereochemistry of the uronic acid and the sulfation pattern on binding. Elimination of the HeLa surface glycosaminoglycans with lyases also resulted in severe adherence impairment. Advantage was taken of the Lactobacillus-glycosaminoglycans interaction to identify an adhesin from the bacterial surface. This protein, identify as a soluble binding protein of an ABC transporter system (OppA) by MALDI-TOF/(MS), was overproduced in Escherichia coli, purified and shown to interfere with L. salivarius Lv72 adhesion to HeLa cells.
conclusionsThese data suggest that glycosaminoglycans play a fundamental role in attachment of mutualistic bacteria to the epithelium that lines the cavities where the normal microbiota thrives, OppA being a bacterial adhesin involved in the process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.