ArticleJournal of ovarian research2013
Ovarian stem cells: absence of evidence is not evidence of absence.
Article in Journal of ovarian research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Can Molecular Attributes of Mammalian Granulosa Cells and Ovarian Putative Stem Cells Predestine Them to Be a Promising Tool for Tissue Engineering and Regenerative Medicine?International journal of molecular sciences · 2025Review
- Overview of follicle stimulating hormone and its receptors in reproduction and in stem cells and cancer stem cells.International journal of biological sciences · 2022Review
- Stem Cells in Adult Mice Ovaries Form Germ Cell Nests, Undergo Meiosis, Neo-oogenesis and Follicle Assembly on Regular Basis During Estrus Cycle.Stem cell reviews and reports · 2021Article
- Actions and Roles of FSH in Germinative Cells.International journal of molecular sciences · 2021Review
- Regenerative Medicine Approaches in Bioengineering Female Reproductive Tissues.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary.In vitro cellular & developmental biology. Animal · 2020Article
- Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness.Stem cell research & therapy · 2019Article
- Stem Cells, Self-Renewal, and Lineage Commitment in the Endocrine System.Frontiers in endocrinology · 2019Review
- Stem cells survive oncotherapy & can regenerate non-functional gonads: A paradigm shift for oncofertility.The Indian journal of medical research · 2018Review
- Ovarian stem cells-resolving controversies.Journal of assisted reproduction and genetics · 2018Article
- Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly.Journal of ovarian research · 2018Article
- Germ stem cells are active in postnatal mouse ovary under physiological conditions.Molecular human reproduction · 2016Article
- The effect of the immune system on ovarian function and features of ovarian germline stem cells.SpringerPlus · 2016Review
- Novel Action of FSH on Stem Cells in Adult Mammalian Ovary Induces Postnatal Oogenesis and Primordial Follicle Assembly.Stem cells international · 2016Review
- Improvement in Isolation and Identification of Mouse Oogonial Stem Cells.Stem cells international · 2016Article
- VSELs may obviate cryobanking of gonadal tissue in cancer patients for fertility preservation.Journal of ovarian research · 2015Article
- Perspective in infertility: the ovarian stem cells.Journal of ovarian research · 2015Article
- Mouse Ovarian Very Small Embryonic-Like Stem Cells Resist Chemotherapy and Retain Ability to Initiate Oocyte-Specific Differentiation.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- Very small embryonic-like stem cells are involved in pancreatic regeneration and their dysfunction with age may lead to diabetes and cancer.Stem cell research & therapy · 2015Review
- Very small embryonic-like stem cells are the elusive mouse endometrial stem cells--a pilot study.Journal of ovarian research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLei and Spradling in a recent study published in PNAS failed to detect 'germline cysts' by elegant studies using lineage tracing approach and thus concluded that adult mouse ovaries lack stem cells. They proposed that primordial follicle pool generated during fetal life is sufficient to sustain oogenesis and that there is no renewal of oocytes during adult life. Contrary to their results, we have reported presence of very small pluripotent, embryonic-like stem cells (VSELs), their immediate descendants (OGSCs) and germ cell 'cysts' or 'nests' (formed by rapid cell division and incomplete cytokinesis) in surface epithelial cell smears of adult sheep, monkey and human ovaries.
methodsIn the present study, ovaries were collected from adult mouse (treated with 5 IU pregnant mare serum gonadotropin, PMSG) and sheep (from slaughter house) and testis from mouse treated with busulphan (25 mg/Kg). Ovarian surface epithelial (OSE) cells and testicular smears were studied for the presence of cysts. Sheep OSE smears were also used to show cytoplasmic continuity amongst the cyst cells studied by immunolocalization and confocal microscopy of stem cells specific markers OCT-4 and SSEA-4.
resultsCysts were observed and confocal microscopy imaging confirmed cytoplasmic continuity amongst the cells comprising the cysts.
conclusionsCysts represent self-renewal and clonal expansion of stem cells with incomplete cytokinesis and are a hallmark feature of stem cells. We suggest the use of PMSG stimulated mouse ovaries and use of more primitive markers like OCT-4 or STELLA rather than MVH for lineage tracing studies to conclusively show presence of stem cells by lineage-tracing studies.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.