ArticleThe American journal of pathology1990
Plasma membrane GPIIb/IIIa. Evidence for a cycling receptor pool.
Article in The American journal of pathology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 59 citations in OpenAlex.
- The WASH-complex subunit Strumpellin regulates integrin αIIbβ3 trafficking in murine platelets.Scientific reports · 2023Article
- New insights of platelet endocytosis and its implication for platelet function.Frontiers in cardiovascular medicine · 2023Review
- Article
- The ins and outs of endocytic trafficking in platelet functions.Current opinion in hematology · 2017Review
- Article
- Bound fibrinogen distribution on stimulated platelets. Examination by confocal scanning laser microscopy.The American journal of pathology · 1995Article
- Absence of ligands bound to glycoprotein IIB-IIIA on the exposed surface of a thrombus may limit thrombus growth in flowing blood.The Journal of clinical investigation · 1994Article
- Cellular distribution of CD63 antigen in platelets and in three megakaryocytic cell lines.The Histochemical journal · 1994Article
- Studies of activated GPIIb/IIIa receptors on the luminal surface of adherent platelets. Paradoxical loss of luminal receptors when platelets adhere to high density fibrinogen.The Journal of clinical investigation · 1993Article
- Kistrin, an integrin antagonist, blocks endocytosis of fibrinogen into guinea pig megakaryocyte and platelet alpha-granules.The Journal of clinical investigation · 1993Article
- Article
- In vivo defibrination results in markedly decreased amounts of fibrinogen in rat megakaryocytes and platelets.The American journal of pathology · 1990Article
- Pharmacodynamic study of F(ab')2 fragments of murine monoclonal antibody 7E3 directed against human platelet glycoprotein IIb/IIIa in patients with unstable angina pectoris.The Journal of clinical investigation · 1990Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
The author used immunofluorescence and digital image processing to investigate the dynamic distribution of GPIIb/IIIa in living platelets. Resting cells were incubated with AP-2, a complex-specific, monoclonal, anti-GPIIb/IIIa antibody. Examination of intact cells demonstrated a rim pattern for GPIIb/IIIa consistent with a surface localization. Permeabilization revealed a time-dependent increase in the labeling of apparent intracellular vacuoles. This pattern is distinct from the "patch-cap" pattern observed when unfixed platelets were incubated with fluoresceinated concanavalin A. Additionally, labeling of this vacuolar pool of GPIIb/IIIa was inhibited by treatment with 2% sodium azide or by incubation at 4 degrees C. Identical staining patterns were obtained with Fab fragments of AP-2. Ultrastructural examination confirmed the presence of labeled intracellular vacuolar structures. Parallel studies performed with AP-1, a monoclonal anti-GPIb antibody, failed to demonstrate internalization of GPIb. Finally, thrombin stimulation of resting platelets, which had been preincubated with AP-2, resulted in the clearing of this newly internalized pool of GPIIb/IIIa; presumably via translocation to the surface. These data suggest the presence of an actively cycling pool of GPIIb/IIIa that has not been described previously. The dynamic distribution of this pool may be important in the regulation of platelet adhesiveness.
Indexed as
Identifiers
2404419PMC1877449W275055882What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.