ArticleJournal of experimental botany2013
LEA polypeptide profiling of recalcitrant and orthodox legume seeds reveals ABI3-regulated LEA protein abundance linked to desiccation tolerance.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 2 of them syntheses that pooled it.
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Who cites it
55 citing papers in PubMed, 2 syntheses or guidelines pooled it, 148 citations in OpenAlex.
- Proline concentrations in seedlings of woody plants change with drought stress duration and are mediated by seed characteristics: a meta-analysis.Scientific reports · 2023Pooled it
- Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives.Frontiers in plant science · 2022Pooled it
- Transcriptional insights into molecular events occurring during tuber maturation of tigernut (Cyperus esculentus L.).Functional & integrative genomics · 2026Article
- Comparative proteomic analysis of desiccation responses in recalcitrant Quercus acutissima seeds.Plant molecular biology · 2025Article
- Integrative Analysis Provides Insights into Genes Encoding LEA_5 Domain-Containing Proteins in Tigernut (Plants (Basel, Switzerland) · 2025Article
- Towards resilience: Transcriptional insights on flavonoid biosynthesis during peanut seed maturation phases.PloS one · 2025Article
- Insights into Genes Encoding LEA_1 Domain-Containing Proteins inPlants (Basel, Switzerland) · 2024Article
- Transcriptomic Profiling of Two Rice Thermo-Sensitive Genic Male Sterile Lines with Contrasting Seed Storability after Artificial Accelerated Aging Treatment.Plants (Basel, Switzerland) · 2024Article
- Shotgun proteomics profiling of chia seeds (Frontiers in plant science · 2024Article
- Analysis and Identification of Genes Associated with the Desiccation Sensitivity ofPlants (Basel, Switzerland) · 2023Article
- The Arabidopsis F-box protein SKP1-INTERACTING PARTNER 31 modulates seed maturation and seed vigor by targeting JASMONATE ZIM DOMAIN proteins independently of jasmonic acid-isoleucine.The Plant cell · 2023Article
- iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance ofPlants (Basel, Switzerland) · 2023Article
- Molecular dynamics of seed priming at the crossroads between basic and applied research.Plant cell reports · 2023Review
- Article
- Snapshot of four mature quinoa (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Chromosome-level genome assembly ofFrontiers in plant science · 2023Article
- New Information of the Anatomy and Phytochemical Screening ofInternational journal of food science · 2023Article
- Chayote Fruit (Plants (Basel, Switzerland) · 2022Article
- Involvement of dehydrin proteins in mitigating the negative effects of drought stress in plants.Plant cell reports · 2022Review
- Molecular Aspects of Seed Development Controlled by Gibberellins and Abscisic Acids.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to orthodox seeds that acquire desiccation tolerance during maturation, recalcitrant seeds are unable to survive drying. These desiccation-sensitive seeds constitute an interesting model for comparative analysis with phylogenetically close species that are desiccation tolerant. Considering the importance of LEA (late embryogenesis abundant) proteins as protective molecules both in drought and in desiccation tolerance, the heat-stable proteome was characterized in cotyledons of the legume Castanospermum australe and it was compared with that of the orthodox model legume Medicago truncatula. RNA sequencing identified transcripts of 16 homologues out of 17 LEA genes for which polypeptides are detected in M. truncatula seeds. It is shown that for 12 LEA genes, polypeptides were either absent or strongly reduced in C. australe cotyledons compared with M. truncatula seeds. Instead, osmotically responsive, non-seed-specific dehydrins accumulated to high levels in the recalcitrant cotyledons compared with orthodox seeds. Next, M. truncatula mutants of the abscisic acid insensitive3 (ABI3) gene were characterized. Mature Mtabi3 seeds were found to be desiccation sensitive when dried below a critical water content of 0.4 g H2O g DW(-1). Characterization of the LEA proteome of the Mtabi3 seeds revealed a subset of LEA proteins with severely reduced abundance that were also found to be reduced or absent in C. australe cotyledons. Transcripts of these genes were indeed shown to be ABI3 responsive. The results highlight those LEA proteins that are critical to desiccation tolerance and suggest that comparable regulatory pathways responsible for their accumulation are missing in both desiccation-sensitive genotypes, revealing new insights into the mechanistic basis of the recalcitrant trait in seeds.
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