ArticleProceedings of the National Academy of Sciences of the United States of America2013
Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74 citing papers in PubMed, 109 citations in OpenAlex.
- Subtype-specific neutralizing antibodies promote antigenic shift during influenza virus co-infection.Virulence · 2026Article
- Divergent pathogenic mechanisms of influenza A and influenza B viruses.Archives of microbiology · 2026Review
- A Structural View of Influenza Virus Ribonucleoprotein Complex and Its Functions.Microorganisms · 2026Review
- Molecular epidemiology and genetic characteristics of influenza viruses in a local pediatric population of eastern China, 2024.Frontiers in microbiology · 2026Article
- Spatiotemporal reconstruction of the North American A(H5N1) outbreak reveals successive lineage replacements by descendant reassortants.Science advances · 2025Article
- Using homologous network to identify reassortment risk in H5Nx avian influenza viruses.PLoS computational biology · 2025Article
- Machine learning methods for predicting human-adaptive influenza A virus reassortment based on intersegment constraint.Frontiers in microbiology · 2025Article
- Mapping Binding Sites of Nucleoprotein Within the Influenza Virus RNA Genome.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Incompatible packaging signals and impaired protein functions hinder reassortment of bat H17N10 or H18N11 segment 7 with human H1N1 influenza A viruses.Journal of virology · 2024Article
- Sequential disruption of SPLASH-identified vRNA-vRNA interactions challenges their role in influenza A virus genome packaging.Nucleic acids research · 2023Article
- Local changes in viral RNA sequence drive global changes in influenza nucleoprotein binding.Journal of medical virology · 2023Article
- Influenza A virus reassortment is strain dependent.PLoS pathogens · 2023Article
- Review
- Genetic and Antigenic Characterization of an Expanding H3 Influenza A Virus Clade in U.S. Swine Visualized by Nextstrain.mSphere · 2022Article
- Genome-Wide Reassortment Analysis of Influenza A H7N9 Viruses Circulating in China during 2013-2019.Viruses · 2022Article
- Study of the host specificity of PB1-F2-associated virulence.Virulence · 2021Article
- Limited onward transmission potential of reassortment genotypes from chickens co-infected with H9N2 and H7N9 avian influenza viruses.Emerging microbes & infections · 2021Article
- Review
- Genome-wide analyses of human noroviruses provide insights on evolutionary dynamics and evidence of coexisting viral populations evolving under recombination constraints.PLoS pathogens · 2021Article
14 more citing papers are in PubMed but not listed here.
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14 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fragmented nature of the influenza A genome allows the exchange of gene segments when two or more influenza viruses infect the same cell, but little is known about the rules underlying this process. Here, we studied genetic reassortment between the A/Moscow/10/99 (H3N2, MO) virus originally isolated from human and the avian A/Finch/England/2051/91 (H5N2, EN) virus and found that this process is strongly biased. Importantly, the avian HA segment never entered the MO genetic background alone but always was accompanied by the avian PA and M fragments. Introduction of the 5' and 3' packaging sequences of HA(MO) into an otherwise HA(EN) backbone allowed efficient incorporation of the chimerical viral RNA (vRNA) into the MO genetic background. Furthermore, forcing the incorporation of the avian M segment or introducing five silent mutations into the human M segment was sufficient to drive coincorporation of the avian HA segment into the MO genetic background. These silent mutations also strongly affected the genotype of reassortant viruses. Taken together, our results indicate that packaging signals are crucial for genetic reassortment and that suboptimal compatibility between the vRNA packaging signals, which are detected only when vRNAs compete for packaging, limit this process.
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