ArticleProceedings of the National Academy of Sciences of the United States of America2013
Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 80 citations in OpenAlex.
- Defining the human lung pathodegradome of the V8 protease fromInfection and immunity · 2026Article
- Spectrin coordinates cell shape and signaling essential for epidermal differentiation.The Journal of cell biology · 2026Article
- Multi-omics identify hallmark protein and lipid features of small extracellular vesicles circulating in human plasma.Nature cell biology · 2025Article
- Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis.Biomolecules · 2025Article
- YAP/TAZ Signalling Controls Epidermal Keratinocyte Fate.International journal of molecular sciences · 2024Review
- Article
- Vaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling.Cell reports · 2023Article
- Genomic instability caused by Arp2/3 complex inactivation results in micronucleus biogenesis and cellular senescence.PLoS genetics · 2023Article
- Anillin governs mitotic rounding during early epidermal development.BMC biology · 2022Article
- Dia1 coordinates differentiation and cell sorting in a stratified epithelium.The Journal of cell biology · 2022Article
- Functions of Arp2/3 Complex in the Dynamics of Epithelial Tissues.Frontiers in cell and developmental biology · 2022Review
- Rac1 promotes kidney collecting duct integrity by limiting actomyosin activity.The Journal of cell biology · 2021Article
- Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation.Cell death and differentiation · 2021Article
- Loss of CD73 shifts transforming growth factor-β1 (TGF-β1) from tumor suppressor to promoter in endometrial cancer.Cancer letters · 2021Article
- The cell pushes back: The Arp2/3 complex is a key orchestrator of cellular responses to environmental forces.Current opinion in cell biology · 2021Review
- The Arp2/3 Inhibitory Protein Arpin Is Required for Intestinal Epithelial Barrier Integrity.Frontiers in cell and developmental biology · 2021Article
- Thymosin β4 is essential for adherens junction stability and epidermal planar cell polarity.Development (Cambridge, England) · 2020Article
- The Arp2/3 complex is crucial for colonisation of the mouse skin by melanoblasts.Development (Cambridge, England) · 2020Article
- Scaling up single-cell mechanics to multicellular tissues - the role of the intermediate filament-desmosome network.Journal of cell science · 2020Review
- Profilin-Mediated Actin Allocation Regulates the Growth of Epithelial Microvilli.Current biology : CB · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The epidermis provides an essential seal from the external environment and retains fluids within the body. To form an effective barrier, cells in the epidermis must form tight junctions and terminally differentiate into cornified envelopes. Here, we demonstrate that the branched actin nucleator, the actin-related protein (Arp)2/3 complex, is unexpectedly required for both these activities. Loss of the ArpC3 subunit of the Arp2/3 complex resulted in minimal changes in the morphogenesis and architecture of this stratified squamous epithelium, but resulted in profound defects in its physiology. Mutant embryos did not develop an effective barrier to the external environment and died within hours of birth. We discovered two underlying causes for these effects. First, ArpC3 was essential for robust assembly and function of tight junctions, specialized cell-cell adhesions that restrict water loss in the epidermis. Second, there were defects in differentiation of the epidermis and the production of cornified envelopes, structures essential for barrier activity. Underlying this defect, we found that YAP was inappropriately active not only in the ArpC3 mutant tissue, but also in cultured cells. Inhibition of YAP activity rescued the differentiation and barrier defects caused by loss of ArpC3. These results demonstrate previously unappreciated roles for the Arp2/3 complex and highlight the functions of branched actin networks in a complex tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.