ArticleProceedings of the National Academy of Sciences of the United States of America2013
Active output state of the Synechococcus Kai circadian oscillator.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Mechanism and reconstitution of circadian transcription in cyanobacteria.Nature structural & molecular biology · 2026Article
- The current situations and limitations of genetic engineering in cyanobacteria: a mini review.Molecular biology reports · 2023Review
- Glycogen metabolism is required for optimal cyanobacterial growth in the rapid light-dark cycle of low-Earth orbit.Life sciences in space research · 2023Article
- Reconstitution of an intact clock reveals mechanisms of circadian timekeeping.Science (New York, N.Y.) · 2021Article
- Orchestration of Circadian Timing by Macromolecular Protein Assemblies.Journal of molecular biology · 2020Review
- A Hard Day's Night: Cyanobacteria in Diel Cycles.Trends in microbiology · 2019Review
- Roles for ClpXP in regulating the circadian clock inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Structural basis of the day-night transition in a bacterial circadian clock.Science (New York, N.Y.) · 2017Article
- Redox crisis underlies conditional light-dark lethality in cyanobacterial mutants that lack the circadian regulator, RpaA.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Combined Computational and Genetic Approach Uncovers Network Interactions of the Cyanobacterial Circadian Clock.Journal of bacteriology · 2016Article
- Toward Multiscale Models of Cyanobacterial Growth: A Modular Approach.Frontiers in bioengineering and biotechnology · 2016Review
- Circadian Rhythms in Cyanobacteria.Microbiology and molecular biology reviews : MMBR · 2015Review
- The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Giving Time Purpose: The Synechococcus elongatus Clock in a Broader Network Context.Annual review of genetics · 2015Review
- High-throughput and quantitative approaches for measuring circadian rhythms in cyanobacteria using bioluminescence.Methods in enzymology · 2015Article
- Metabolic design for cyanobacterial chemical synthesis.Photosynthesis research · 2014Review
- Article
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4 authors.
Funding
Abstract
The mechanisms by which cellular oscillators keep time and transmit temporal information are poorly understood. In cyanobacteria, the timekeeping aspect of the circadian oscillator, composed of the KaiA, KaiB, and KaiC proteins, involves a cyclic progression of phosphorylation states at Ser431 and Thr432 of KaiC. Elucidating the mechanism that uses this temporal information to modulate gene expression is complicated by unknowns regarding the number, structure, and regulatory effects of output components. To identify oscillator signaling states without a complete description of the output machinery, we defined a simple metric, Kai-complex output activity (KOA), that represents the difference in expression of reporter genes between strains that carry specific variants of KaiC and baseline strains that lack KaiC. In the absence of the oscillator, expression of the class 1 paradigm promoter P(kaiBC) was locked at its usual peak level; conversely, that of the class 2 paradigm promoter P(purF) was locked at its trough level. However, for both classes of promoters, peak KOA in wild-type strains coincided late in the circadian cycle near subjective dawn, when KaiC-pST becomes most prevalent (Ser431 is phosphorylated and Thr432 is not). Analogously, peak KOA was detected specifically for the phosphomimetic of KaiC-pST (KaiC-ET). Notably, peak KOA required KaiB, indicating that a KaiBC complex is involved in the output activity. We also found evidence that phosphorylated RpaA (regulator of phycobilisome associated) represses an RpaA-independent output of KOA. A simple mathematical expression successfully simulated two key features of the oscillator-the time of peak KOA and the peak-to-trough amplitude changes.
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