ArticleDiabetes2013
In vivo adeno-associated viral vector-mediated genetic engineering of white and brown adipose tissue in adult mice.
Article in Diabetes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
58 citing papers in PubMed, 89 citations in OpenAlex.
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- Cars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- MicroRNA-based targeting strategies enable AAV-mediated, tissue-selective gene expression in adipose tissue and skeletal muscle of mice.Molecular therapy. Advances · 2026Article
- Netrin-1 disrupt high-fat-diet-induced adipogenesis via the PPARγ and Wnt/β-catenin signaling pathways.Communications biology · 2026Article
- Interleukin-10 expressing B lineage cells in visceral adipose tissue protect against aging-related insulin resistance and extend lifespan.Nature communications · 2026Article
- Dual Role of Transcription Factors in the Development and Thermogenic Function of Brown Adipose Tissue.International journal of medical sciences · 2026Review
- Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin.Signal transduction and targeted therapy · 2025Article
- Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue.Nature communications · 2025Article
- The Long Noncoding RNA ΒFaar Promotes White Adipose Tissue Browning and Prevents Diet-Induced Obesity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry.Biology · 2025Article
- A distal enhancer with ETV4 binding is critical for UCP1 expression and thermogenesis in brown fat.Genes & development · 2025Article
- IL-27 alleviates high-fat diet-induced obesity and metabolic disorders by inhibiting adipogenesis via activating HDAC6.Communications biology · 2025Article
- SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice.Cell death and differentiation · 2025Article
- Article
- An efficient AAV vector system of Rec2 serotype for intravenous injection to study metabolism in brown adipocytes in vivo.Molecular metabolism · 2024Article
- Assessment of Adipocyte Transduction Using Different AAV Capsid Variants.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Preclinical evaluation of tissue-selective gene therapies for congenital generalised lipodystrophy.Gene therapy · 2024Article
- Efficient AAV9 Purification Using a Single-Step AAV9 Magnetic Affinity Beads Isolation.International journal of molecular sciences · 2024Article
- Vestigial like 4 regulates the adipogenesis of classical brown adipose tissue.bioRxiv : the preprint server for biology · 2024Article
- Identification of circulating apolipoprotein M as a new determinant of insulin sensitivity and relationship with adiponectin.International journal of obesity (2005) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adipose tissue is pivotal in the regulation of energy homeostasis through the balance of energy storage and expenditure and as an endocrine organ. An inadequate mass and/or alterations in the metabolic and endocrine functions of adipose tissue underlie the development of obesity, insulin resistance, and type 2 diabetes. To fully understand the metabolic and molecular mechanism(s) involved in adipose dysfunction, in vivo genetic modification of adipocytes holds great potential. Here, we demonstrate that adeno-associated viral (AAV) vectors, especially serotypes 8 and 9, mediated efficient transduction of white (WAT) and brown adipose tissue (BAT) in adult lean and obese diabetic mice. The use of short versions of the adipocyte protein 2 or uncoupling protein-1 promoters or micro-RNA target sequences enabled highly specific, long-term AAV-mediated transgene expression in white or brown adipocytes. As proof of concept, delivery of AAV vectors encoding for hexokinase or vascular endothelial growth factor to WAT or BAT resulted in increased glucose uptake or increased vessel density in targeted depots. This method of gene transfer also enabled the secretion of stable high levels of the alkaline phosphatase marker protein into the bloodstream by transduced WAT. Therefore, AAV-mediated genetic engineering of adipose tissue represents a useful tool for the study of adipose pathophysiology and, likely, for the future development of new therapeutic strategies for obesity and diabetes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.