ArticleThe Journal of cell biology2013
Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging.
Article in The Journal of cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 52 citations in OpenAlex.
- Confinement energy landscape classification reveals membrane receptor nano-organization mechanisms.Biophysical journal · 2024Article
- Zeolite-iron oxide integrated interdigitated electrode sensor for diagnosing cervical cancer.Heliyon · 2024Article
- Silicon-Based Scaffold for Wound Healing Skin Regeneration Applications: A Concise Review.Polymers · 2022Review
- Applications of Nanotechnology in Sensor-Based Detection of Foodborne Pathogens.Sensors (Basel, Switzerland) · 2020Review
- Multiplex Photoluminescent Silicon Nanoprobe for Diagnostic Bioimaging and Intracellular Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Silicon Nanomaterials for Biosensing and Bioimaging Analysis.Frontiers in chemistry · 2018Review
- Silicon Nanocrystals with pH-Sensitive Tunable Light Emission from Violet to Blue-Green.Sensors (Basel, Switzerland) · 2017Article
- Recent Advances in Silicon Nanomaterial-Based Fluorescent Sensors.Sensors (Basel, Switzerland) · 2017Review
- Tracking single molecules at work in living cells.Nature chemical biology · 2014Review
- The More Exotic Shapes of Semiconductor Nanocrystals: Emerging Applications in Bioimaging.Current opinion in chemical engineering · 2014Article
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence microscopy is used extensively in cell-biological and biomedical research, but it is often plagued by three major problems with the presently available fluorescent probes: photobleaching, blinking, and large size. We have addressed these problems, with special attention to single-molecule imaging, by developing biocompatible, red-emitting silicon nanocrystals (SiNCs) with a 4.1-nm hydrodynamic diameter. Methods for producing SiNCs by simple chemical etching, for hydrophilically coating them, and for conjugating them to biomolecules precisely at a 1:1 ratio have been developed. Single SiNCs neither blinked nor photobleached during a 300-min overall period observed at video rate. Single receptor molecules in the plasma membrane of living cells (using transferrin receptor) were imaged for ≥10 times longer than with other probes, making it possible for the first time to observe the internalization process of receptor molecules at the single-molecule level. Spatial variations of molecular diffusivity in the scale of 1-2 µm, i.e., a higher level of domain mosaicism in the plasma membrane, were revealed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.