ArticleThe Journal of biological chemistry2013
Characterization of the nucleoid-associated protein YejK.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Structural Studies on the M. tuberculosis Nucleoid-associated-Protein, NapA, Indicates DNA Bridging Mechanism.Journal of molecular biology · 2025Article
- Fur-regulated urease contributes to the environmental adaptation ofMicrobiology spectrum · 2025Article
- Structure of the E. coli nucleoid-associated protein YejK reveals a novel DNA binding clamp.Nucleic acids research · 2024Article
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Variation of Structure and Cellular Functions of Type IA Topoisomerases across the Tree of Life.Cells · 2024Review
- High-resolution temporal profiling of E. coli transcriptional response.Nature communications · 2023Article
- StaR Is a Positive Regulator of Topoisomerase I Activity Involved in Supercoiling Maintenance inInternational journal of molecular sciences · 2023Article
- Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.Microbiology spectrum · 2023Article
- Variable DNA topology is an epigenetic generator of physiological heterogeneity in bacterial populations.Molecular microbiology · 2023Review
- Pangenome-level analysis of nucleoid-associated proteins in theFrontiers in microbiology · 2023Article
- Extended sister-chromosome catenation leads to massive reorganization of the E. coli genome.Nucleic acids research · 2022Article
- Proteomic Analyses ofFrontiers in cellular and infection microbiology · 2021Article
- Topoisomerase III Acts at the Replication Fork To Remove Precatenanes.Journal of bacteriology · 2019Article
- Endogenous and Foreign Nucleoid-Associated Proteins of Bacteria: Occurrence, Interactions and Effects on Mobile Genetic Elements and Host's Biology.Computational and structural biotechnology journal · 2019Review
- Article
- The MukB-topoisomerase IV interaction is required for proper chromosome compaction.The Journal of biological chemistry · 2017Article
- Comparative genomic analyses reveal a vast, novel network of nucleotide-centric systems in biological conflicts, immunity and signaling.Nucleic acids research · 2015Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nucleoid-associated proteins play an important role in condensing chromosomal DNA and regulating gene expression. We report here the characterization of the nucleoid-associated protein YejK, which was detected in a yeast two-hybrid screen using the ParE subunit of topoisomerase IV as bait. The purified protein likely exists in a monomer-dimer equilibrium in solution and can form tetramers. Cross-linking of the protein bound to DNA suggests that the active form could be either a dimer or tetramer. YejK, which is present at about 24,000 copies of monomer per mid-log phase cell, binds double-stranded DNA with a site size of 12-14 base pairs/monomer, does not display a significant preference for either bent compared with straight DNA or supercoiled compared with relaxed DNA, and untwists DNA somewhat as it binds. YejK binds RNA, but not single-stranded DNA, with 65% of the avidity with which it binds DNA. However, cells deleted for yejK do not show defects in either RNA or protein synthesis. YejK interacts with all the subunits of both DNA gyrase and topoisomerase IV and has measurable effects on their activities. In the presence of YejK, relaxation of negatively supercoiled DNA by topoisomerase IV becomes distributive, whereas relaxation of positively supercoiled DNA is stimulated. Relaxation of negatively supercoiled DNA by DNA gyrase is inhibited, whereas the extent of supercoiling of relaxed DNA is limited. A YejK-GFP chimera is an effective marker for the nucleoid in live cell imaging.
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