ArticleEnvironmental science and pollution research international2014
Standard inocula preparations reduce the bacterial diversity and reliability of regulatory biodegradation tests.
Article in Environmental science and pollution research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Influence of temperature and inoculum composition on standardized biodegradation tests of bioplastics in freshwater under aerobic conditions.Scientific reports · 2026Article
- Persistence Assessment of Chemicals: Trajectories toward New Approach Methodologies (P-NAMs).Environmental science & technology · 2026Review
- Testing the biodegradability of difficult compounds: a future challenge for the OECD/ISO standardization.Applied microbiology and biotechnology · 2026Review
- High-Throughput Miniaturized Biotransformation Testing Using Activated Sludge Enables Rapid Chemical Persistence Assessment.Environmental science & technology letters · 2025Article
- Biodegradation assessment tests of biopolymers in standardised water: different sources of variability.Biodegradation · 2025Article
- Biodegradation of Water-Soluble Polymers by Wastewater Microorganisms: Challenging Laboratory Testing Protocols.Environmental science & technology · 2024Article
- Toward the future of OECD/ISO biodegradability testing-new approaches and developments.Applied microbiology and biotechnology · 2023Review
- Scientific concepts and methods for moving persistence assessments into the 21st century.Integrated environmental assessment and management · 2022Review
- Biotransformation of Chemicals at the Water-Sediment Interface-Toward a Robust Simulation Study Setup.ACS environmental Au · 2021Article
- Improving our understanding of the environmental persistence of chemicals.Integrated environmental assessment and management · 2021Article
- Improving the ecological relevance of aquatic bacterial communities in biodegradability screening assessments.The Science of the total environment · 2018Article
- Biodegradability standards for carrier bags and plastic films in aquatic environments: a critical review.Royal Society open science · 2018Review
- From laboratory to environmental conditions: a new approach for chemical's biodegradability assessment.Environmental science and pollution research international · 2016Article
- Evaluating the ready biodegradability of two poorly water-soluble substances: comparative approach of bioavailability improvement methods (BIMs).Environmental science and pollution research international · 2016Article
- Characterization of para-Nitrophenol-Degrading Bacterial Communities in River Water by Using Functional Markers and Stable Isotope Probing.Applied and environmental microbiology · 2015Article
- Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
OECD ready biodegradability tests have been central to understanding the biodegradation of chemicals from a regulatory perspective for many decades. They are not fit for contemporary prioritisation of chemicals based on persistence, however, due to the low concentration of inocula used, short duration and high variability between tests. Two OECD standard inoculum pretreatment methods (settlement and filtration) were investigated to observe their effect on the probability of biodegradation and associated changes in bacterial community structure and diversity of inocula sourced from the activated sludge process of wastewater treatment plants. Both settlement and filtration were shown to dramatically and significantly reduce the probability and increase the variability of biodegradation of 4-nitrophenol compared to the use of unprocessed inocula. These differences were associated with a significant hundred-fold reduction in cell numbers and solids content and a significant shift in bacterial community structure that was sometimes accompanied by significant reductions in detectable operational taxonomic unit richness and evenness. The natural variation (between different environments) and variation due to differential selection of bacterial communities (by different pretreatment methods) is offered as an explanation for the historical high variability in standard OECD ready biodegradability tests.
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