ArticleThe Journal of antimicrobial chemotherapy2014
Regulation of acrAB expression by cellular metabolites in Escherichia coli.
Article in The Journal of antimicrobial chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
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Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- Expression of multidrug efflux pump gene acrAB in Escherichia coli: a systematic review and meta analysis.BMC infectious diseases · 2025Pooled it
- How resistance-nodulation-division (RND) efflux pumps contribute to virulence.PLoS pathogens · 2026Review
- Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026Review
- Characterization of anAntibiotics (Basel, Switzerland) · 2026Article
- RND-mediated efflux couples antimicrobial resistance and hypervirulence in contemporary Vibrio cholerae.PLoS pathogens · 2026Article
- Antibiotic resistance inFrontiers in microbiology · 2026Article
- Global transcriptomics and targeted metabolite analysis reveal the involvement of the AcrAB efflux pump in physiological functions by exporting signaling molecules inMicrobiology spectrum · 2025Article
- Systematic screen uncovers regulator contributions to chemical cues in Escherichia coli.PLoS biology · 2025Article
- Determining the potential targets of silybin by molecular docking and its antibacterial functions on efflux pumps and porins in uropathogenic E. coli.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Disruption of the EmrAB-TolC efflux pump ofmicroPublication biology · 2025Article
- The complex development of psoralen-interstrand crosslink resistance inbioRxiv : the preprint server for biology · 2024Article
- Dual role ofiMetaOmics · 2024Article
- Physiological Effects of TolC-Dependent Multidrug Efflux Pumps in Escherichia coli: Impact on Motility and Growth Under Stress Conditions.MicrobiologyOpen · 2024Article
- Antimicrobial Resistance in Livestock: A Serious Threat to Public Health.Antibiotics (Basel, Switzerland) · 2024Review
- Fitness trade-offs of multidrug efflux pumps inApplied and environmental microbiology · 2024Article
- Multidrug resistance ofmBio · 2024Article
- The induced and intrinsic resistance ofMicrobiology spectrum · 2024Article
- Article
- Functional analysis of bacterial genes accidentally packaged in rhizospheric phageome of the wild plant speciesSaudi journal of biological sciences · 2023Article
- Abundant resistome determinants in rhizosphere soil of the wild plant Abutilon fruticosum.AMB Express · 2023Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
objectivesMultidrug efflux pumps mediate resistance to antibiotics and other toxic compounds. We studied the role of AcrAB-TolC, the main efflux pump in Escherichia coli, in regulating gene expression.
methodsDeletion mutants, an acrABp-lacZ fusion and reverse transcription-real-time quantitative PCR experiments were used to study the role of AcrAB-TolC and metabolism in regulating gene expression of the acrAB operon and its transcriptional regulators.
resultsDeletion of the acrB gene increased the expression of the acrAB operon. A similar induction of acrAB was found when acrA or tolC was deleted, and when the pump function was inhibited using phenylalanine-arginine-β-naphthylamide. The induction of acrAB in the ΔacrB strain was totally (AcrR or SoxS) or partially (SoxR or MarA) prevented when the genes for these acrAB regulators were also deleted. The expression of soxS and marA, but not of acrR, was increased in the ΔacrB strain, which also showed altered expression of many other genes related to different cellular processes, including motility. Deletion of the metabolic genes entA and entE (enterobactin biosysnthesis), glpX (gluconeogenesis), cysH (cysteine biosynthesis) and purA (purine biosynthesis) also prevented activation of the acrAB promoter in the ΔacrB strain. Addition of the enterobactin biosynthesis intermediate metabolite 2,3-dihydroxybenzoate induced the expression of acrAB.
conclusionsThese results together suggest a model in which the AcrAB-TolC pump effluxes cellular metabolites that are toxic and/or have a signalling role. If the pump is inactivated or inhibited, these metabolites would accumulate, inactivating AcrR and/or up-regulating soxS and marA expression, ultimately triggering the up-regulation of acrAB expression to restore homeostasis.
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