ArticleSpringerPlus2013
Bias problems in culture-independent analysis of environmental bacterial communities: a representative study on hydrocarbonoclastic bacteria.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Identification of bacteria associated with canine otitis externa based on 16S rDNA high-throughput sequencing.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Investigation of bacterial and fungal population structure on environmental surfaces of three medical institutions during the COVID-19 pandemic.Frontiers in microbiology · 2023Article
- Effect of dilution factor on the isolation ofIranian journal of microbiology · 2022Article
- Spring is associated with increased total and allergenic fungal concentrations in house dust from a pediatric asthma cohort in New York City.Building and environment · 2022Article
- Further culture-independent characterization of the lactic microbiota of Serro artisanal cheese.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- The Rhizosphere Microbiome of Ginseng.Microorganisms · 2022Review
- Associations between self-reported psychological symptom severity and gut microbiota: further support for the microgenderome.BMC psychiatry · 2022Article
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- Prokaryotic and eukaryotic microbial diversity from three soda lakes in the East African Rift Valley determined by amplicon sequencing.Frontiers in microbiology · 2022Article
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- Vaginal Microbiota of the Sexually Transmitted Infections Caused byMicroorganisms · 2021Article
- Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures.Biology · 2021Article
- Diversity and Oil Degradation Potential of Culturable Microbes Isolated from Chronically Contaminated Soils in Trinidad.Microorganisms · 2021Article
- Microbiome Compositions From Infertile Couples SeekingFrontiers in cellular and infection microbiology · 2021Article
- Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil.Frontiers in microbiology · 2021Review
- Characterization of Chlorella vulgaris (Trebouxiophyceae) associated microbial communitiesJournal of phycology · 2020Article
- Revealing the secret life of skin - with the microbiome you never walk alone.International journal of cosmetic science · 2020Review
- Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence.Frontiers in plant science · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture-dependent methods for bacterial community analysis are currently considered obsolete; therefore, molecular techniques are usually used instead. The results of the current study on hydrocarbonoclastic bacteria in various oily habitats in Kuwait showed however, that the bacterial identities varied dramatically according to the analytical approach used. For six desert and six seawater samples used in this study, the culture-independent and culture-dependent techniques each led to a unique bacterial composition. Problems related to the culture-dependent technique are well known. The results of the current study highlighted bias problems other than those already recorded in the literature for the molecular approaches. Thus, for example, in contrast to the culture-dependent technique, the primers used in the molecular approach preferentially amplified the 16S rDNAs of hydrocarbonoclastic bacteria in total genomic DNAs of all the studied environmental samples, and in addition, failed to reveal in any environmental sample members of the Actinobacteria. The primers used in the molecular approach also amplified certain "pure" 16S rDNAs, but failed to do so when these DNAs were in mixture. In view of these results, it is recommended that the two analytical approaches should be used simultaneously because their combined results would reflect the bacterial community composition more precisely than either of them can do alone.
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Registered trials
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