ArticleJournal of periodontal & implant science2013
The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells.
Article in Journal of periodontal & implant science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Coaxial electrospinning of poly(ɛ-caprolactone)/gelatin core-shell biodegradable implants for localized delivery of metronidazole and dexamethasone for periodontal applications.Frontiers in bioengineering and biotechnology · 2026Article
- A cellular automaton model of osteogenic differentiation reveals identifiability limits of endpoint assays.Frontiers in systems biology · 2026Article
- Cyclodextrins as Multi-Functional Ingredients in Dentistry.Pharmaceutics · 2023Review
- Effect of vitamin DJournal of periodontal research · 2021Article
- Electrospinning of dexamethasone/cyclodextrin inclusion complex polymer fibers for dental pulp therapy.Colloids and surfaces. B, Biointerfaces · 2020Article
- Hypoxia Helps Maintain Nucleus Pulposus Homeostasis by Balancing Autophagy and Apoptosis.Oxidative medicine and cellular longevity · 2020Article
- Acute psychological stress increases serum circulating cell-free mitochondrial DNA.Psychoneuroendocrinology · 2019Article
- Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications.Nanomaterials (Basel, Switzerland) · 2019Review
- Bone Tissue Engineering in a Perfusion Bioreactor Using Dexamethasone-Loaded Peptide Hydrogel.Materials (Basel, Switzerland) · 2019Article
- Valproic acid prevents glucocorticoid‑induced osteonecrosis of the femoral head of rats.International journal of molecular medicine · 2018Article
- Are We Economically Efficient Enough to Increase the Potential ofThe open orthopaedics journal · 2016Article
- In vitro Osteogenic impulse effect of Dexamethasone on periodontal ligament stem cells.Bioinformation · 2015Article
- Anti-hepatoma cells function of luteolin through inducing apoptosis and cell cycle arrest.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Influence of Titanium Surface Treatments on Viability of Periodontal Fibroblasts Grown in an Osteogenic Culture Medium.Contemporary clinical dentistryArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of the current study was to examine the effect of dexamethasone (Dex) at various concentrations on the apoptosis and mineralization of human periodontal ligament (hPDL) cells.
methodshPDL cells were obtained from the mid-third of premolars extracted for orthodontic reasons, and a primary culture of hPDL cells was prepared using an explant technique. Groups of cells were divided according to the concentration of Dex (0, 1, 10, 100, and 1,000 nM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed for evaluation of cellular viability, and alkaline phosphatase activity was examined for osteogenic differentiation of hPDL cells. Alizarin Red S staining was performed for observation of mineralization, and real-time polymerase chain reaction was performed for the evaluation of related genes.
resultsIncreasing the Dex concentration was found to reduce cellular viability, with an increase in alkaline phosphatase activity and mineralization. Within the range of Dex concentrations tested in this study, 100 nM of Dex was found to promote the most vigorous differentiation and mineralization of hPDL cells. Dex-induced osteogenic differentiation and mineralization was accompanied by an increase in the level of osteogenic and apoptosis-related genes and a reduction in the level of antiapoptotic genes. The decrease in hPDL cellular viability by glucocorticoid may be explained in part by the increased prevalence of cell apoptosis, as demonstrated by BAX expression and decreased expression of the antiapoptotic gene, Bcl-2.
conclusionsAn increase in hPDL cell differentiation rather than cellular viability at an early stage is likely to be a key factor in glucocorticoid induced mineralization. In addition, apoptosis might play an important role in Dex-induced tissue regeneration; however, further study is needed for investigation of the precise mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.