ArticlePloS one2013
A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
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- DPP4 inhibition impairs senohemostasis to improve plaque stability in atherosclerotic mice.The Journal of clinical investigation · 2023Article
- Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme.Nature communications · 2023Article
- CD38 plays an age-related role in cholinergic deregulation of airway smooth muscle contractility.The Journal of allergy and clinical immunology · 2022Article
- Oscillating calcium signals in smooth muscle cells underlie the persistent basal tone of internal anal sphincter.Journal of cellular physiology · 2021Article
- Mode Switch of CaFrontiers in physiology · 2021Article
- Preserving Airway Smooth Muscle Contraction in Precision-Cut Lung Slices.Scientific reports · 2020Article
- Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering.Stem cell reports · 2019Article
- Smooth muscle: a stiff sculptor of epithelial shapes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
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- Augmented Pla2g4c/Ptgs2/Hpgds axis in bronchial smooth muscle tissues of experimental asthma.PloS one · 2018Article
- March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation.Journal of immunology research · 2018Article
- Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life.Mucosal immunology · 2016Article
- Disruption of miR-29 Leads to Aberrant Differentiation of Smooth Muscle Cells Selectively Associated with Distal Lung Vasculature.PLoS genetics · 2015Article
- An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Phenotypes of lung smooth muscle cells in health and disease are poorly characterized. This is due, in part, to a lack of methodologies that allow for the independent and direct isolation of bronchial smooth muscle cells (BSMCs) and vascular smooth muscle cells (VSMCs) from the lung. In this paper, we describe the development of a bi-fluorescent mouse that permits purification of these two cell populations by cell sorting. By subjecting this mouse to an acute allergen based-model of airway inflammation that exhibits many features of asthma, we utilized this tool to characterize the phenotype of so-called asthmatic BSMCs. First, we examined the biophysical properties of single BSMCs from allergen sensitized mice and found increases in basal tone and cell size that were sustained ex vivo. We then generated for the first time, a comprehensive characterization of the global gene expression changes in BSMCs isolated from the bi-fluorescent mice with allergic airway inflammation. Using statistical methods and pathway analysis, we identified a number of differentially expressed mRNAs in BSMCs from allergen sensitized mice that code for key candidate proteins underlying changes in matrix formation, contractility, and immune responses. Ultimately, this tool will provide direction and guidance for the logical development of new markers and approaches for studying human lung smooth muscle.
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Registered trials
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