ArticlePloS one2013
Single cell synchrotron FT-IR microspectroscopy reveals a link between neutral lipid and storage carbohydrate fluxes in S. cerevisiae.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Characterization of lipid droplets from a Taxus media cell suspension and their potential involvement in trafficking and secretion of paclitaxel.Plant cell reports · 2022Article
- Biophysical Stress Responses of the YeastFrontiers in microbiology · 2020Article
- Exposure ofFrontiers in microbiology · 2019Article
- Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide inFrontiers in microbiology · 2019Article
- PUX10 Is a CDC48A Adaptor Protein That Regulates the Extraction of Ubiquitinated Oleosins from Seed Lipid Droplets in Arabidopsis.The Plant cell · 2018Article
- Metabolic engineering of lipid pathways in Saccharomyces cerevisiae and staged bioprocess for enhanced lipid production and cellular physiology.Journal of industrial microbiology & biotechnology · 2018Article
- The Peroxygenase Activity of theFrontiers in microbiology · 2018Article
- Single cell assessment of yeast metabolic engineering for enhanced lipid production using Raman and AFM-IR imaging.Biotechnology for biofuels · 2018Article
- Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples.Frontiers in microbiology · 2017Review
- Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 β-Aggregated Structures in Influenza A Virus-infected Cells.The Journal of biological chemistry · 2016Article
- Using mid infrared technology as new method for the determination of the dwell time of salivary substitutes on three dimensional gingiva models.BMC ear, nose, and throat disorders · 2016Article
- Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes.Oxidative medicine and cellular longevity · 2016Article
- Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems.Protein and peptide letters · 2016Article
- Physiological and biochemical responses of Yarrowia lipolytica to dehydration induced by air-drying and freezing.PloS one · 2014Article
- Development of a univariate membrane-based mid-infrared method for protein quantitation and total lipid content analysis of biological samples.Journal of analytical methods in chemistry · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most organisms, storage lipids are packaged into specialized structures called lipid droplets. These contain a core of neutral lipids surrounded by a monolayer of phospholipids, and various proteins which vary depending on the species. Hydrophobic structural proteins stabilize the interface between the lipid core and aqueous cellular environment (perilipin family of proteins, apolipoproteins, oleosins). We developed a genetic approach using heterologous expression in Saccharomyces cerevisiae of the Arabidopsis thaliana lipid droplet oleosin and caleosin proteins AtOle1 and AtClo1. These transformed yeasts overaccumulate lipid droplets, leading to a specific increase in storage lipids. The phenotype of these cells was explored using synchrotron FT-IR microspectroscopy to investigate the dynamics of lipid storage and cellular carbon fluxes reflected as changes in spectral fingerprints. Multivariate statistical analysis of the data showed a clear effect on storage carbohydrates and more specifically, a decrease in glycogen in our modified strains. These observations were confirmed by biochemical quantification of the storage carbohydrates glycogen and trehalose. Our results demonstrate that neutral lipid and storage carbohydrate fluxes are tightly connected and co-regulated.
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