ArticlePloS one2013
Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Genomic Repertoire of Twenty-Two Novel Vibrionaceae Species Isolated from Marine Sediments.Microbial ecology · 2025Article
- The Tol Pal system integrates maintenance of the three layered cell envelope.npj antimicrobials and resistance · 2024Review
- The genome of a hadal sea cucumber reveals novel adaptive strategies to deep-sea environments.iScience · 2022Article
- Characterization of the Chimeric PriB-SSBc Protein.International journal of molecular sciences · 2021Article
- Oxidation of trimethylamine to trimethylamineScience advances · 2021Article
- High-pressure small-angle X-ray scattering cell for biological solutions and soft materials.Journal of applied crystallography · 2021Article
- The multifarious roles of Tol-Pal in Gram-negative bacteria.FEMS microbiology reviews · 2020Review
- Roles of the DedD Protein inJournal of bacteriology · 2019Article
- Increases of heat shock proteins and their mRNAs at high hydrostatic pressure in a deep-sea piezophilic bacterium, Shewanella violacea.Extremophiles : life under extreme conditions · 2015Article
- Article
- Escherichia coli genes and pathways involved in surviving extreme exposure to ionizing radiation.Journal of bacteriology · 2014Article
- Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.Nucleic acids research · 2014Article
- Enzyme recruitment and its role in metabolic expansion.Biochemistry · 2014Review
- Adaptive laboratory evolution of Escherichia coli K-12 MG1655 for growth at high hydrostatic pressure.Frontiers in microbiology · 2014Article
- Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.BioMed research international · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the fact that much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood. We use a genome-wide screening approach, combined with a novel high-throughput high-pressure cell culture method, to investigate the effects of hydrostatic pressure on microbial physiology in vivo. The Keio collection of single-gene deletion mutants in Escherichia coli K-12 was screened for growth at a range of pressures from 0.1 MPa to 60 MPa. This led to the identification of 6 genes, rodZ, holC, priA, dnaT, dedD and tatC, whose products were required for growth at 30 MPa and a further 3 genes, tolB, rffT and iscS, whose products were required for growth at 40 MPa. Our results support the view that the effects of pressure on cell physiology are pleiotropic, with DNA replication, cell division, the cytoskeleton and cell envelope physiology all being potential failure points for cell physiology during growth at elevated pressure.
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