ArticlePloS one2013
Role and regulation of the Flp/Tad pilus in the virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 50 citations in OpenAlex.
- Type IV pilin regulation: a transcriptional overview.Critical reviews in microbiology · 2026Review
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- Genetic Determinants and Biofilm Properties Useful in Estimation of UTI Pathogenicity of theAntibiotics (Basel, Switzerland) · 2025Article
- Impact of Homologous Recombination on Core Genome Evolution and Host Adaptation of Pectobacterium parmentieri.Genome biology and evolution · 2024Article
- Genetic Loci of Plant PathogenicInternational journal of molecular sciences · 2024Article
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- Tad pili contribute to the virulence and biofilm formation of virulentFrontiers in cellular and infection microbiology · 2024Article
- In-Silico Analysis Highlights the Existence in Members ofMicroorganisms · 2023Article
- Identification and characterization of opportunistic pathogenFrontiers in plant science · 2023Article
- Quantitative Real-Time PCR Assay for the Detection ofPlants (Basel, Switzerland) · 2021Article
- The First Polish Isolate of a Novel SpeciesInternational journal of environmental research and public health · 2021Article
- Genome-Wide Analyses of the Temperature-Responsive Genetic Loci of the Pectinolytic Plant PathogenicInternational journal of molecular sciences · 2021Article
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- Genome-Wide Identification ofFrontiers in plant science · 2020Article
- Transcriptome profiling ofAccess microbiology · 2020Article
- Genetic Variants of the DSF Quorum Sensing System inFrontiers in microbiology · 2020Article
- Genome-Wide Analyses Revealed Remarkable Heterogeneity in Pathogenicity Determinants, Antimicrobial Compounds, and CRISPR-Cas Systems of Complex Phytopathogenic GenusPathogens (Basel, Switzerland) · 2019Article
- Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens.MicrobiologyOpen · 2019Article
- A stealth adhesion factor contributes to Vibrio vulnificus pathogenicity: Flp pili play roles in host invasion, survival in the blood stream and resistance to complement activation.PLoS pathogens · 2019Article
- Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we characterized a putative Flp/Tad pilus-encoding gene cluster, and we examined its regulation at the transcriptional level and its role in the virulence of potato pathogenic enterobacteria of the genus Pectobacterium. The Flp/Tad pilus-encoding gene clusters in Pectobacterium atrosepticum, Pectobacterium wasabiae and Pectobacterium aroidearum were compared to previously characterized flp/tad gene clusters, including that of the well-studied Flp/Tad pilus model organism Aggregatibacter actinomycetemcomitans, in which this pilus is a major virulence determinant. Comparative analyses revealed substantial protein sequence similarity and open reading frame synteny between the previously characterized flp/tad gene clusters and the cluster in Pectobacterium, suggesting that the predicted flp/tad gene cluster in Pectobacterium encodes a Flp/Tad pilus-like structure. We detected genes for a novel two-component system adjacent to the flp/tad gene cluster in Pectobacterium, and mutant analysis demonstrated that this system has a positive effect on the transcription of selected Flp/Tad pilus biogenesis genes, suggesting that this response regulator regulate the flp/tad gene cluster. Mutagenesis of either the predicted regulator gene or selected Flp/Tad pilus biogenesis genes had a significant impact on the maceration ability of the bacterial strains in potato tubers, indicating that the Flp/Tad pilus-encoding gene cluster represents a novel virulence determinant in Pectobacterium. Soft-rot enterobacteria in the genera Pectobacterium and Dickeya are of great agricultural importance, and an investigation of the virulence of these pathogens could facilitate improvements in agricultural practices, thus benefiting farmers, the potato industry and consumers.
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